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| Status |
Public on Apr 09, 2010 |
| Title |
Expression data from undifferentiated human pluripotent stem cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Due to their somatic cell origin, human induced pluripotent stem cells (HiPSCs) are assumed to carry a normal diploid genome, and adaptive chromosomal aberrations have not been fully evaluated. Here, we analyzed the chromosomal integrity of 66 HiPSC and 38 human embryonic stem cell (HESC) samples from 18 different studies by global gene expression meta-analysis. We report identification of a substantial number of cell lines carrying full and partial chromosomal aberrations, half of which were validated at the DNA level. Several aberrations resulted from culture adaptation, and others are suspected to originate from the parent somatic cell. Our classification revealed a third type of aneuploidy already evident in early passage HiPSCs, suggesting considerable selective pressure during the reprogramming process. The analysis indicated high incidence of chromosome 12 duplications, resulting in significant enrichment for cell cycle related genes. Such aneuploidy may limit the differentiation capacity and increase the tumorigenicity of HiPSCs.
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| Overall design |
Expression data from undifferentiated human embryonic stem cells and induced pluripotent stem cells
total RNA was isolated from undifferentiated pluripotent stem cells grown in standard HESC growth conditions on mouse embryonic fibroblast feeder layer.
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| Contributor(s) |
Mayshar Y, Ben-David U, Bar-Nur O, Pick M, Yanuka O, Darr H, Benvenisty N |
| Citation(s) |
20887957 |
| Submission date |
Apr 07, 2010 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Uri Ben-David |
| E-mail(s) |
uri.bendavid@gmail.com
|
| Phone |
97226585185
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| Organization name |
Hebrew University
|
| Department |
Genetics
|
| Lab |
Nissim Benvenisty
|
| Street address |
Givat-Ram
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| City |
Jerusalem |
| ZIP/Postal code |
91904 |
| Country |
Israel |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (7)
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| GSM531019 |
undifferentiated H9 Human embryonic stem cells replicate 1 |
| GSM531020 |
undifferentiated H9 Human embryonic stem cells replicate 2 |
| GSM531021 |
undifferentiated H9 Human embryonic stem cells replicate 3 |
| GSM531022 |
undifferentiated BJ-TERT derived induced pluripotent stem cell line 28 replicate 2 |
| GSM531023 |
undifferentiated BJ-TERT derived induced pluripotent stem cell line 28 replicate 1 |
| GSM531024 |
undifferentiated primary foreskin fibroblast derived induced pluripotent stem cell line 1 |
| GSM531025 |
undifferentiated BJ-TERT derived induced pluripotent stem cell line 29 |
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| This SubSeries is part of SuperSeries: |
| GSE21262 |
Expression data from undifferentiated and induced human pluripotent stem cells |
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| Relations |
| BioProject |
PRJNA129717 |
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