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| Status |
Public on May 20, 2010 |
| Title |
Comparison of expression profiles of once and three time linearly amplified samples |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
In order to assess the impact of three rounds of linear amplification on the technical reproducibility of gene expression measurements, we performed twelve microarray experiments. We analysed mouse RNA from cortex, cerebellum and liver from one individual. One RNA sample of 5µg from each of the three different tissues was processed according to the standard Affymetrix protocol and hybridized onto mouse gene expression arrays MG_U74Av2. Three additional samples from each tissue of 1ng were processed according to a modified procedure that involves three linear amplifications before hybridization onto the microarray chips.
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| Overall design |
One mouse was analysed. One RNA sample of 5µg from each of liver, cortex, and cerebellum were processed according to the standard Affymetrix protocol and hybridized onto mouse gene expression arrays MG_U74Av2. Three additional samples (technical replicates) from each tissue of 1ng were processed according to a modified procedure that involves three linear amplifications before hybridization onto the microarray chips, resulting in 12 microarray experiments.
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| Contributor(s) |
Giger T, Khaitovich P, Somel M, Lorenc A, Lizano E, Harris LW, Ryan MM, Lan M, Wayland MT, Bahn S, Pääbo S |
| Citation(s) |
20624733 |
| Submission date |
Apr 19, 2010 |
| Last update date |
Feb 18, 2018 |
| Contact name |
Thomas Giger |
| E-mail(s) |
giger@eva.mpg.de
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| Organization name |
Max-Planck-Institute of Evolutionary Anthropology
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| Department |
Evolutionary Genetics
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| Street address |
Deutscher Platz 6
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| City |
Leipzig |
| ZIP/Postal code |
04106 |
| Country |
Germany |
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| Platforms (1) |
| GPL81 |
[MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA126057 |
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