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Series GSE21493 Query DataSets for GSE21493
Status Public on Nov 01, 2018
Title Gene expression study in skin fibroblasts
Organism Mus musculus
Experiment type Expression profiling by array
Summary To investigate the in vivo developmental potential, we generated dedifferentiated-skin fibroblast from adult skin fibroblasts with a FVB background, a different genetic strain from the protein-donor embryonic stem (ES) cell. Primary cardiac fibroblast (cFB) was cultered from an adult C57/BL mouse to compare with ES cells(E14-mES, C57-mES). We also introduced C57 background ES cell-derived extract proteins into cFB by reversible permeabilisation. The ES-like cells (colonies) were expanded with subculture every 3 to 5 days using the standard ES cell culture protocol.
 
Overall design Total RNA was extracted from each sample. Five-hundred nanograms of total RNA were used for labeling and hybridization, according to the manufacturer’s protocols (Illumina). After the bead chips were scanned with an Illumina BeadArray Reader (Illumina), the expression level of each gene was transformed into a log 2 base before further analysis.
 
Contributor(s) Leem SH, Kim SK, Chu IS
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Submission date Apr 23, 2010
Last update date Nov 01, 2018
Contact name Seon-Kyu Kim
E-mail(s) seonkyu@kribb.re.kr
Organization name Korea Research Institutue of Bioscience & Biotechnology
Department Personalized Genomic Medicine Research Center
Street address 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Korea
City Daejeon
ZIP/Postal code 305-806
Country South Korea
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (20)
GSM537092 E14-mES.1
GSM537093 E14-mES.2
GSM537094 E14-mES.3
Relations
BioProject PRJNA126017

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE21493_RAW.tar 3.1 Mb (http)(custom) TAR
GSE21493_nonnormalized.txt.gz 2.1 Mb (ftp)(http) TXT
Processed data included within Sample table
Raw data are available on Series record

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