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Series GSE21539 Query DataSets for GSE21539
Status Public on Apr 28, 2010
Title Comparison of expression data between control and Ovo1 morphant zebrafish embryos
Organism Danio rerio
Experiment type Expression profiling by array
Summary A fundamental issue in cell biology is how migratory cell behaviors are controlled by dynamically regulated cell adhesion.
Vertebrate neural crest (NC) cells rapidly alter cadherin expression and localization at the cell surface during migration. Secreted Wnts induce some of these changes in NC adhesion and also promote specification of NC-derived pigment cells. Here, we show that the zebrafish transcription factor Ovo1 is a Wnt target gene that controls migration of pigment precursors by regulating the intracellular movements of N-cadherin (Ncad). Ovo1 genetically interacts with Ncad and its depletion causes Ncad to accumulate inside cells. Ovo1-deficient embryos strongly upregulate factors involved in intracellular trafficking, including several rab GTPases, known to modulate cellular localization of cadherins. Surprisingly, NC cells express high levels of many of these rab genes in the early embryo, chemical inhibitors of Rab functions rescue NC development in Ovo1-deficient embryos and overexpression of a Rab-interacting protein leads to similar defects in NC migration. These results suggest that Ovo proteins link Wnt signaling to intracellular trafficking pathways that localize Ncad in NC cells and allow them to migrate. Similar processes probably occur in other cell types in which Wnt signaling promotes migration.
Overall design Total RNA from control and Ovo1 morphant, sox10(7.2):gfp transgenic
embryos was isolated using Trizol reagent (Gibco/BRL) from 12 hpf embryos, when morphants first show NC defects. Experiments were performed in triplicate. RNA samples were processed as per manufacturer’s instructions (Affymetrix GeneChip Expression Analysis Technical Manual, Affymetric, Inc., Santa Clara, CA, USA).
Contributor(s) Piloto S, Schilling TF
Citation(s) 20463035
Submission date Apr 27, 2010
Last update date Jan 25, 2018
Contact name Sarah Piloto
Organization name University of California, Irvine
Department Developmental and Cell Biology
Lab Thomas Schilling
Street address 4462 Natural Sciences II, Dept. Dev. and Cell Biology
City Irvine
State/province CA
ZIP/Postal code 92697
Country USA
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (6)
GSM537962 zebrafish 12hpf control-1
GSM537963 zebrafish 12hpf control-2
GSM537964 zebrafish 12hpf control-3
BioProject PRJNA126199

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE21539_RAW.tar 13.3 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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