NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE216409 Query DataSets for GSE216409
Status Public on Oct 25, 2022
Title The Nup98::Nsd1 fusion gene induces CD123 expression in 32D cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary The NUP98::NSD1 fusion gene is associated with extremely poor prognosis in patients with acute myeloid leukemia (AML). NUP98::NSD1 induces self-renewal and blocks differentiation of hematopoietic stem cells, leading to leukemia development. Despite its association with poor prognosis, targeted therapy for NUP98::NSD1-positive AML is lacking, as the details of NUP98::NSD1 function are unknown. Here, we generated 32D cells (a murine interleukin-3 (IL-3)-dependent myeloid progenitor cell line) expressing mouse Nup98::Nsd1 to explore the function of NUP98::NSD1 in AML, including by comprehensive gene expression analysis. We identified two properties of Nup98::Nsd1+ 32D cells in vitro: first, Nup98::Nsd1 promoted blocking of AML cell differentiation, consistent with a previous report; second, Nup98::Nsd1 increased dependence on IL-3 for cell proliferation, due to overexpression of the alpha subunit of the IL-3 receptor (IL3-RA, also known as CD123). Consistent with our in vitro data, IL3-RA was also up-regulated in samples from patients with NUP98::NSD1-positive AML. These results highlight CD123 as a potential new therapeutic target in NUP98::NSD1-positive AML.
 
Overall design We compared gene expression profiling of Nup98::Nsd1+ 32D and mock-transduced 32D cells using a GeneChip™ Mouse Genome 430 2.0 Array to explore the function of NUP98::NSD1 in AML, including by comprehensive gene expression analysis.
 
Contributor(s) Okamoto K, Imamura T, Tanaka S, Yoshida H, Shiba N, Iehara T
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Oct 24, 2022
Last update date Oct 28, 2022
Contact name Kenji Okamoto
E-mail(s) kokamoto@koto.kpu-m.ac.jp
Phone 090-7481-6247
Organization name Department of Pediatrics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine
Street address 465, Kajii-cho, Kawaramachi-Hirokoji, Kamigyo-ku
City Kyoto
State/province Kyoto
ZIP/Postal code 602-8566
Country Japan
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (6)
GSM6672089 32D_MSCVpuro_1
GSM6672090 32D_MSCVpuro_2
GSM6672091 32D_MSCVpuro_3
Relations
BioProject PRJNA893565

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE216409_RAW.tar 24.7 Mb (http)(custom) TAR (of CEL, CHP, TXT)
Processed data included within Sample table
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap