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GEO help: Mouse over screen elements for information. |
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Status |
Public on Oct 25, 2022 |
Title |
The Nup98::Nsd1 fusion gene induces CD123 expression in 32D cells |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
The NUP98::NSD1 fusion gene is associated with extremely poor prognosis in patients with acute myeloid leukemia (AML). NUP98::NSD1 induces self-renewal and blocks differentiation of hematopoietic stem cells, leading to leukemia development. Despite its association with poor prognosis, targeted therapy for NUP98::NSD1-positive AML is lacking, as the details of NUP98::NSD1 function are unknown. Here, we generated 32D cells (a murine interleukin-3 (IL-3)-dependent myeloid progenitor cell line) expressing mouse Nup98::Nsd1 to explore the function of NUP98::NSD1 in AML, including by comprehensive gene expression analysis. We identified two properties of Nup98::Nsd1+ 32D cells in vitro: first, Nup98::Nsd1 promoted blocking of AML cell differentiation, consistent with a previous report; second, Nup98::Nsd1 increased dependence on IL-3 for cell proliferation, due to overexpression of the alpha subunit of the IL-3 receptor (IL3-RA, also known as CD123). Consistent with our in vitro data, IL3-RA was also up-regulated in samples from patients with NUP98::NSD1-positive AML. These results highlight CD123 as a potential new therapeutic target in NUP98::NSD1-positive AML.
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Overall design |
We compared gene expression profiling of Nup98::Nsd1+ 32D and mock-transduced 32D cells using a GeneChip™ Mouse Genome 430 2.0 Array to explore the function of NUP98::NSD1 in AML, including by comprehensive gene expression analysis.
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Contributor(s) |
Okamoto K, Imamura T, Tanaka S, Yoshida H, Shiba N, Iehara T |
Citation missing |
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Submission date |
Oct 24, 2022 |
Last update date |
Oct 28, 2022 |
Contact name |
Kenji Okamoto |
E-mail(s) |
kokamoto@koto.kpu-m.ac.jp
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Phone |
090-7481-6247
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Organization name |
Department of Pediatrics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine
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Street address |
465, Kajii-cho, Kawaramachi-Hirokoji, Kamigyo-ku
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City |
Kyoto |
State/province |
Kyoto |
ZIP/Postal code |
602-8566 |
Country |
Japan |
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Platforms (1) |
GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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Samples (6)
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Relations |
BioProject |
PRJNA893565 |
Supplementary file |
Size |
Download |
File type/resource |
GSE216409_RAW.tar |
24.7 Mb |
(http)(custom) |
TAR (of CEL, CHP, TXT) |
Processed data included within Sample table |
Processed data provided as supplementary file |
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