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| Status |
Public on Dec 01, 2022 |
| Title |
Effects of Chronic Social Stress on the transcriptome of BA-NAc neurons |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Reduced reward interest/learning and reward-to-effort valuation are distinct, common symptoms in neuropsychiatric disorders for which chronic stress is a major aetiological factor. Pyramidal glutamate neurons in the basal amygdala (BA) project to various brain regions including nucleus accumbens (NAc). The BA-NAc neural pathway is activated by reward and aversion, with many neurons being monovalent. In adult male mice, chronic social stress (CSS) led to both reduced discriminative reward learning (DRL) associated with decreased BA-NAc Ca2+ activity, and reduced sucrose reward-to-effort valuation (REV) associated, in contrast, with increased BA-NAc Ca2+ activity. Chronic tetanus toxin inhibition of BA-NAc neurons replicated the CSS-DRL effect whilst causing only a mild REV reduction, whilst chronic DREADDs activation of BA-NAc neurons replicated the CSS effect on REV without affecting DRL. This study provides novel evidence that chronic stress disruption of reward processing involves the BA-NAc neural pathway; the bi-directional effects implicate activity changes in BA-NAc reward (learning) and aversion (effort) neurons, with the net overall direction of stress-induced change in activity dependent on on-going stimulus processing and behaviour.
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| Overall design |
Assessment of the effects of chronic social stress on the transcriptome of BA-NAc "reward-sensitive" glutamatergic neurons when compared to BA-Nac glutamatergic neurons from control non-stressed animals.
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| Contributor(s) |
Madur L, Viollet C, Alanis-Lobato G, Pryce CR |
| Citation(s) |
37061616 |
| Submission date |
Oct 26, 2022 |
| Last update date |
May 12, 2023 |
| Contact name |
Gregorio Alanis-Lobato |
| E-mail(s) |
g.alanis.lobato@gmail.com
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| Organization name |
Boehringer Ingelheim Pharma
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| Department |
Global Computational Biology and Digital Sciences
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| Street address |
Birkendorfer Str 65
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| City |
Biberach an der Riss |
| ZIP/Postal code |
88400 |
| Country |
Germany |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (33)
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| GSM6680921 |
BA-NAc neurons, CSS, rep2 |
| GSM6680922 |
BA-NAc neurons, Control, rep3 |
| GSM6680923 |
BA-NAc neurons, Control, rep4 |
| GSM6680924 |
BA-NAc neurons, CSS, rep3 |
| GSM6680925 |
BA-NAc neurons, CSS, rep4 |
| GSM6680926 |
BA-NAc neurons, Control, rep5 |
| GSM6680927 |
BA-NAc neurons, Control, rep6 |
| GSM6680928 |
BA-NAc neurons, CSS, rep5 |
| GSM6680929 |
BA-NAc neurons, CSS, rep6 |
| GSM6680930 |
BA-NAc neurons, CSS, rep7 |
| GSM6680931 |
BA-NAc neurons, CSS, rep8 |
| GSM6680932 |
BA-NAc neurons, CSS, rep9 |
| GSM6680933 |
BA-NAc neurons, CSS, rep10 |
| GSM6680934 |
BA-NAc neurons, CSS, rep11 |
| GSM6680935 |
BA-NAc neurons, CSS, rep12 |
| GSM6680936 |
BA-NAc neurons, Control, rep7 |
| GSM6680937 |
BA-NAc neurons, Control, rep8 |
| GSM6680938 |
BA-NAc neurons, CSS, rep13 |
| GSM6680939 |
BA-NAc neurons, CSS, rep14 |
| GSM6680940 |
BA-NAc neurons, CSS, rep15 |
| GSM6680941 |
BA-NAc neurons, CSS, rep16 |
| GSM6680942 |
BA-NAc neurons, Control, rep9 |
| GSM6680943 |
Amygdala tissue, no CTB neurons, rep1 |
| GSM6680944 |
Amygdala tissue, CTB neurons, rep1 |
| GSM6680945 |
Amygdala tissue, no CTB neurons, rep2 |
| GSM6680946 |
Amygdala tissue, CTB neurons, rep2 |
| GSM6680947 |
Amygdala tissue, no CTB neurons, rep3 |
| GSM6680948 |
Amygdala tissue, CTB neurons, rep3 |
| GSM6680949 |
Amygdala tissue, no CTB neurons, rep4 |
| GSM6680950 |
Amygdala tissue, CTB neurons, rep4 |
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| Relations |
| BioProject |
PRJNA894461 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE216587_2020_1011_CNS_CSS_BA-NAc_reward_neuron_exp_I_Pryce_SE.rds.gz |
9.0 Mb |
(ftp)(http) |
RDS |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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