|
| Status |
Public on May 10, 2010 |
| Title |
Gene expression data of interleukin-7-dependent and BCR-ABL1-transformed pre-B cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
This analysis focused on identifying factors that protect pre-B cells against DNA double strand break (DSB)-mediated DNA damage stress during pre-B cell differentiation. Differentiation of pre-B cells including immunoglobulin light chain gene recombination were performed by withdrawal of interleukin-7 (IL-7) from IL-7-dependent murine pre-B cells or by inhibition of the BCR-ABL1 kinase activity in BCR-ABL1-transformed pre-B cells.
The BCR-ABL1 kinase inhibitor STI571 (Imatinib) was used for the inhibition of BCR-ABL1.
|
| |
|
| Overall design |
IL-7-dependent murine pre-B cells were either cultured in IL-7 (10 ng/ml) or induced to differentiate by withdrawal of IL-7. BCR-ABL1-transformed pre-B cells were either treated with 10 µM STI571 (in absence or presence of 10 ng/ml IL-7) for 16 hours or cultured without STI571. Three samples for each condition were processed.
|
| |
|
| Contributor(s) |
Duy C, Yu JJ, Nahar R, Swaminathan S, Kweon S, Polo JM, Valls E, Klemm L, Shojaee S, Cerchietti L, Schuh W, Jäck H, Hurtz C, Ramezani-Rad P, Herzog S, Jumaa H, Koeffler PH, de Alborán IM, Melnick AM, Ye HB, Müschen M |
| Citation(s) |
20498019 |
| Submission date |
May 04, 2010 |
| Last update date |
May 04, 2018 |
| Contact name |
Markus Müschen |
| E-mail(s) |
mmuschen@chla.usc.edu
|
| Phone |
+ 1 323 361 5592
|
| Fax |
+1 323 361 1516
|
| URL |
http://www.lymphocytes.de/
|
| Organization name |
University of Southern California
|
| Department |
Childrens Hospital Los Angeles
|
| Lab |
Müschen Lab
|
| Street address |
4650 Sunset Blvd
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90027 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL8321 |
[Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array |
|
| Samples (15)
|
| GSM540647 |
IL-7-dependent pre-B cells - withdrawal of IL-7 rep 1 |
| GSM540648 |
IL-7-dependent pre-B cells - withdrawal of IL-7 rep 2 |
| GSM540649 |
IL-7-dependent pre-B cells - withdrawal of IL-7 rep 3 |
| GSM540650 |
IL-7-dependent pre-B cells rep 1 |
| GSM540651 |
IL-7-dependent pre-B cells rep 2 |
| GSM540652 |
IL-7-dependent pre-B cells rep 3 |
| GSM540653 |
BCR-ABL1-transformed pre-B cells rep 1 |
| GSM540654 |
BCR-ABL1-transformed pre-B cells rep 2 |
| GSM540655 |
BCR-ABL1-transformed pre-B cells rep 3 |
| GSM540680 |
BCR-ABL1-transformed pre-B cells - STI571 treated rep 1 |
| GSM540681 |
BCR-ABL1-transformed pre-B cells - STI571 treated rep 2 |
| GSM540682 |
BCR-ABL1-transformed pre-B cells - STI571 treated rep 3 |
| GSM540683 |
BCR-ABL1-transformed pre-B cells - STI571 treated in presence of IL-7 rep 1 |
| GSM540684 |
BCR-ABL1-transformed pre-B cells - STI571 treated in presence of IL-7 rep 2 |
| GSM540685 |
BCR-ABL1-transformed pre-B cells - STI571 treated in presence of IL-7 rep 3 |
|
| Relations |
| BioProject |
PRJNA125901 |
Less...
More...