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Series GSE21686 Query DataSets for GSE21686
Status Public on Aug 23, 2010
Title Comparison of thymic epithelial cells and hair follicle stem cells
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary Thymic epithelial cells (TECs) are essential for thymopoiesis and form a complex three-dimensional network, the organization of which is strikingly different from other epithelia. Interestingly, TECs express simple epithelia keratins in the cortex, stratified epithelia keratins in the medulla and epidermal differentiation markers in Hassall's bodies. Here we investigate the relationship between thymic epithelium and epidermal differentiation and show that the thymus of the rat contains a population of clonogenic TECs that can be extensively cultured and cloned using conditions developed for epidermal cell therapy in human. Clonogenic TECs conserve a thymic identity and the capacity to integrate in a thymic epithelial network, but they acquire new functionalities when exposed to an inductive skin microenvironment, permanently adopting the fate of hair follicle multipotent stem cells. This change in fate, maintained over time in serial transplantation, correlates with a down-regulation of transcription factors important for thymic identity, and an up-regulation of epidermal markers. Consequently, the TECs’ capacity to integrate in a thymic epithelial network is altered or even lost. Our results demonstrate that the thymus contains a population of holoclone-like epithelial cells that can function as bona fide multipotent keratinocyte stem cells, and that microenvironmental cues are sufficient to re-direct epithelial-cell fate, allowing crossing of primitive germ layer boundaries from endoderm to ectoderm.

Keywords: Cell type comparison
 
Overall design Expression profile difference between hair follicle clones and TECs before and after transplatation
 
Contributor(s) Bonfanti P, Claudinot S, Amici A, Farley A, Blackburn C, Barrandon Y, Pradervand S, Hagenbüchle O
Citation(s) 20725041
Submission date May 05, 2010
Last update date Nov 06, 2013
Contact name Sylvain Pradervand
E-mail(s) Sylvain.Pradervand@unil.ch
Phone +41 21 692 39 08
Organization name UNI Lausanne
Department CIG
Lab DNA Array Facility
Street address Genopode
City Lausanne
ZIP/Postal code 1015
Country Switzerland
 
Platforms (1)
GPL6247 [RaGene-1_0-st] Affymetrix Rat Gene 1.0 ST Array [transcript (gene) version]
Samples (32)
GSM541028 PRE16cl11_VII_rep1
GSM541029 PRE16cl11_VII_rep2
GSM541030 PRP1cl11_VII_rep1
Relations
BioProject PRJNA125849

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE21686_RAW.tar 124.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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