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Series GSE21707 Query DataSets for GSE21707
Status Public on Nov 01, 2011
Title Identification of cardiomyocyte-derived microvesicles content.
Organism Mus musculus
Experiment type Expression profiling by array
Summary Investigation of content of microvesicles exocytosed by cardiomyocytes. The aims of the study were to identify DNA and RNA content in microvesicles from cardiomyocytes.
DNA and RNA were purified from microveicles from cultured cardiomyocytes. Illumina microarrays were used to detect DNA and mRNA molecules and identify them.
 
Overall design Gene expression microarray were used to detect 50 nucleotide long illumina probe sequences in the sequences of the extracted microvesicular DNA.
Terminal deoxynucleotidyl transferase were used to add a poly_T tail to DNA from microvesicles to construct cDNA, possible to label as cRNA and use on the chip to identify part of the sequence of the original DNA.
The microvesicular mRNA content was compared to cardiomyocyte mRNA content (the source of the microvesicles and therefore also the source of microvesicle mRNA).
Two replicates of mRNA from cardiomyocytes and two replicates of mRNA from microvesicles and one sample of DNA from microvesicles were analyzed.
 
Contributor(s) Waldenström A, Hellman U, Ronquist G
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Submission date May 06, 2010
Last update date Jun 14, 2018
Contact name Urban Hellman
E-mail(s) urban.hellman@medbio.umu.se
Phone +46907851621
Organization name Umea University
Department Public health and clinical medicine
Street address Nus 6M
City Umea
ZIP/Postal code 90185
Country Sweden
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (5)
GSM541659 Cardiomyocyte microvesicle_mRNA content_rep1
GSM541660 Cardiomyocyte microvesicle_mRNA content_rep2
GSM541661 Cardiomyocyte microvesicle_DNA content_only one rep.
Relations
BioProject PRJNA127157

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE21707_RAW.tar 3.1 Mb (http)(custom) TAR
GSE21707_non-normalized.txt.gz 1.7 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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