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Series GSE21754 Query DataSets for GSE21754
Status Public on May 13, 2010
Title Expression data from white adipose tissue of Perilipin A transgenic mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary Perilipin A (PeriA) exclusively locates on adipocyte lipid droplets and is essential for lipid storage and lipolysis. Adipocyte specific overexpression of PeriA caused resistance to diet-induced obesity and resulted in improved insulin sensitivity. In order to better understand the biological basis for this observed phenotype we performed DNA microarray analysis on white adipose tissue (WAT) from PeriA transgenic (Tg) and control wildtype (WT) mice.
Overall design We generated transgenic mice that overexpressed human PeriA using the adipocyte specific aP2 promoter/enhancer (Miyoshi, et al. J Lipid Res 2010). All PeriA Tg mice used for the study were female, and heterozygous for the transgene. Littermates that lacked the transgene were used as controls (WT). All mice were housed at room temperature, maintained on a 12 h light/dark cycle, given free access to water, and fed a high-fat diet (HFD) until the age of 30 weeks. On the day prior to tissue harvest at 30 weeks, WAT from perigonadal were rapidly dissected out, extracted total RNA, and hybridized on Affymetrix microarrays.
Contributor(s) Miyoshi H, Sawada T
Citation(s) 21103377
Submission date May 09, 2010
Last update date Feb 11, 2019
Contact name Hideaki Miyoshi
Organization name Hokkaido University Graduate School of Medicine
Department Internal Medicine II
Street address Kita-15, Nishi-7, Kita-ku
City Sapporo
State/province Hokkaido
ZIP/Postal code 060-8638
Country Japan
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (2)
GSM542344 Control_WT_mice_WAT
GSM542345 PeriA_Tg_mice_WAT
BioProject PRJNA127323

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE21754_RAW.tar 12.0 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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