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Series GSE21764 Query DataSets for GSE21764
Status Public on Nov 10, 2010
Title To determine the global gene expression response in murine LPS-induced macrophages treated with a bromodomain inhibitor.
Organism Mus musculus
Experiment type Expression profiling by array
Summary Bone marrow-derived macrophages, Unstimulated DMSO Bone marrow-derived macrophages, Unstimulated + I-BET (GSK525762A) Bone marrow-derived macrophages, LPS 1h DMSO Bone marrow-derived macrophages, LPS 1h + I-BET (GSK525762A) Bone marrow-derived macrophages, LPS 2h DMSO Bone marrow-derived macrophages, LPS 2h + I-BET (GSK525762A) Bone marrow-derived macrophages, LPS 4h DMSO Bone marrow-derived macrophages, LPS 4h + I-BET (GSK525762A)
LPS (100 ng/mL) was purchased from Sigma. Bone Marrow-derived macrophages (BMDMs) were differentiated from C57BL/6 bone marrow using 5 ng/mL each of recombinant M-CSF and IL-3 (Peprotech) for 7 days as described (Jeffrey et al, Nature Immunology, 2006). 2 x10^6 BMDMs were treated with DMSO or 1 μM of I-BET for 30 minutes before the addition of LPS (100 ng/mL) for 1, 2 or 4h. Unstimulated control samples were incubated with I-BET only for 1 hour. 500 ng of total RNA from 3 independent samples per group was used to prepare biotin-labeled RNA using Ambion Illumina TotalPrep RNA Amplification Kit (Applied Biosystems) and hybridized to Illumina MouseRef-8 v2.0 expression BeadChip kits. The chips were scanned using Illumina BeadArray Reader.
 
Overall design 3 biological replicates and 4 timepoints
 
Contributor(s) Jeffrey K, Tarakhovsky A
Citation(s) 21068722
Submission date May 10, 2010
Last update date Jun 14, 2018
Contact name Paul A Wilson
E-mail(s) paul.a.wilson@gsk.com
Organization name GSK
Department Target Sciences
Lab Computational Biology
Street address GlaxoSmithKline Medicine Research Centre
City Stevenage
State/province Hertfordshire
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (24)
GSM542464 0hr_LPS_control_rep1
GSM542465 0hr_LPS_BET treat_rep1
GSM542466 1hr_LPS_control_rep1
Relations
BioProject PRJNA127281

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE21764_RAW.tar 3.1 Mb (http)(custom) TAR
GSE21764_non-normalized.txt.gz 2.8 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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