NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE21768 Query DataSets for GSE21768
Status Public on Nov 25, 2010
Title Klf4 gene disruption is linked to enhanced cellular proliferation and tumor formation
Organism Mus musculus
Experiment type Expression profiling by array
Summary Kruppel-like factor 4 (KLF4) is involved in diverse biological processes such as cell cycle control, differentiation, transcriptional regulation, DNA repair and apoptosis. Furthermore, KLF4 is a tumor suppressor and play a role in regulating genomic stability. In order to investigate the changes in gene expression in the klf4 null MEFs, we compared the gene expression patterns of the wild type and klf4 null by microarray.
 
Overall design Total RNA isolated from Klf4 -/- MEFs compared to Klf4 +/+ MEFs. RNA was processed from cells that had reached 80-90 confluency in triplicate using Trizol reagent as recommended by the manufacturer (Invitrogen, Carlsbad, CA). RNA was subjected to DNase I treatment in order to remove any contaminating genomic DNA. Final purification was performed on RNAeasy columns (Qiagen, Valencia, CA), according to the manufacturer's recommendations.
 
Contributor(s) Hagos E
Citation(s) 21892412
Submission date May 10, 2010
Last update date Mar 09, 2020
Contact name Engda Hagos
E-mail(s) ehagos@emory.edu
Phone 404-7125-8415
Fax 404-727-5767
Organization name Emory University
Department Digestive Diseases
Lab Vincent Yang
Street address 615 Michael Street
City Atlanta
State/province GA
ZIP/Postal code 30322
Country USA
 
Platforms (1)
GPL6105 Illumina mouse-6 v1.1 expression beadchip
Samples (6)
GSM542523 Wild type MEFs Replicate 1
GSM542524 Wild type MEFs Replicate 2
GSM542525 Wild type MEFs Replicate 3
Relations
BioProject PRJNA127289

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE21768_RAW.tar 5.2 Mb (http)(custom) TAR
GSE21768_non-normalized.txt.gz 2.3 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap