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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Sep 07, 2010 |
| Title |
Genome-wide mapping of RNA Pol-II promoter usage in mouse tissues by ChIP-seq |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We report active promoters in five adult mouse tissues - brain, kidney, liver, lung and spleen using ChIP-Seq aganist RNAP-II antibody. We identified 38,639 active RNAP-II transcribed promoters, including 12,270 novel promoters. Of these, 6384 promoters are tissue specific which are CpG poor and contain multiple core promoter elements. By identifying the RNAP-II bound promoter(s) we found that 37% of the protein coding genes use alternative promoters in the five mouse tissues. Roughly 34% of the novel promoters are loacted in CpG-islands suggesting that novel promoters are mostly tissue specific.
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| Overall design |
Study of active promoters in five adult mouse tissues
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| Contributor(s) |
Sun H, Wu J, Wickramasinghe P, Pal S, Gupta R, Bhattachrayya A, Agosto-Perez FJ, Showe LC, Huang TH, Davuluri RV |
| Citation(s) |
20843783 |
| Submission date |
May 11, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Ravi Gupta |
| E-mail(s) |
rgupta@wistar.org
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| Phone |
215-495-6837
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| Fax |
215-495-6847
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| Organization name |
The Wistar Institute
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| Department |
Center for Systems and Computational Biology, Molecular and Cellular Oncogenesis Program
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| Lab |
Davuluri Lab
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| Street address |
3601 Spruce Street
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL9185 |
Illumina Genome Analyzer (Mus musculus) |
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| Samples (5)
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| Relations |
| SRA |
SRP002444 |
| BioProject |
PRJNA127295 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE21773_RAW.tar |
766.0 Mb |
(http)(custom) |
TAR (of BED, TXT, WIG) |
SRA Run Selector |
| Processed data provided as supplementary file |
| Raw data are available in SRA |
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