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| Status |
Public on Jan 19, 2023 |
| Title |
Cellular responses to long-term phosphate starvation of fission yeast: Maf1 determines fate choice between quiescence and death associated with aberrant tRNA biogenesis (CHX experiments) |
| Organism |
Schizosaccharomyces pombe |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
This RNA-Seq analysis compares gene expression of fission yeast cells (Schizosaccharomyces pombe) at various times after phosphate starvation (2, 4, and 8 HR) either with 100 µg/ml cycloheximide or without drug contrasting to the cells grown in phosphate replete conditions (0HR) in the absence of the drug.
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| Overall design |
6 test samples (cycloheximide or no drug treated phosphate starved cells) were analyzed; 1 control sample (no drug, phosphate replete cells); 3 biological replicate for each sample were processed
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| Contributor(s) |
Garg A, Shuman S, Schwer B |
| Citation(s) |
36794724 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R35 GM126945 |
Mechanisms of DNA and RNA transactions |
Sloan Kettering Institute for Cancer Research |
Stewart H Shuman |
| R01 GM134021 |
Inositol pyrophosphate dynamics affect RNA 3'-processing/transcription termination |
WEILL MEDICAL COLLEGE OF CORNELL UNIVERSITY |
BEATE SCHWER |
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| Submission date |
Nov 14, 2022 |
| Last update date |
Apr 20, 2023 |
| Contact name |
Angad Garg |
| Organization name |
Memorial Sloan Kettering Cancer Center
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| Department |
Molecular Biology
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| Lab |
Stewart Shuman
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| Street address |
RRL-865 430 E 67th St.
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| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (1) |
| GPL28961 |
Illumina NovaSeq 6000 (Schizosaccharomyces pombe) |
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| Samples (21)
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| GSM6731090 |
cells; 2h phosphate starved; biol_rep1 |
| GSM6731091 |
cells; 2h phosphate starved; biol_rep2 |
| GSM6731092 |
cells; 2h phosphate starved; biol_rep3 |
| GSM6731093 |
cells; 4h phosphate starved; biol_rep1 |
| GSM6731094 |
cells; 4h phosphate starved; biol_rep2 |
| GSM6731095 |
cells; 4h phosphate starved; biol_rep3 |
| GSM6731096 |
cells; 8h phosphate starved; biol_rep1 |
| GSM6731097 |
cells; 8h phosphate starved; biol_rep2 |
| GSM6731098 |
cells; 8h phosphate starved; biol_rep3 |
| GSM6731099 |
cells; 2h phosphate starved with cycloheximide; biol_rep1 |
| GSM6731100 |
cells; 2h phosphate starved with cycloheximide; biol_rep2 |
| GSM6731101 |
cells; 2h phosphate starved with cycloheximide; biol_rep3 |
| GSM6731102 |
cells; 4h phosphate starved with cycloheximide; biol_rep1 |
| GSM6731103 |
cells; 4h phosphate starved with cycloheximide; biol_rep2 |
| GSM6731104 |
cells; 4h phosphate starved with cycloheximide; biol_rep3 |
| GSM6731105 |
cells; 8h phosphate starved with cycloheximide; biol_rep1 |
| GSM6731106 |
cells; 8h phosphate starved with cycloheximide; biol_rep2 |
| GSM6731107 |
cells; 8h phosphate starved with cycloheximide; biol_rep3 |
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| This SubSeries is part of SuperSeries: |
| GSE217953 |
Cellular responses to long-term phosphate starvation of fission yeast: Maf1 determines fate choice between quiescence and death associated with aberrant tRNA biogenesis |
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| Relations |
| BioProject |
PRJNA901456 |
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