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| Status |
Public on Oct 01, 2010 |
| Title |
Gene expression analysis of IL-4 primed bone marrow macrophages isolated from Irf4-deficient or heterozygous mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To analyse the Irf4-dependent transcriptional changes of mouse bone marrow-derived macrophages (BMM) in response to IL-4, we have employed whole genome microarray expression profiling. For this purpose, bone marrow cells were isolated from 8 to 12 weeks old Irf4-deficient or heterozygous mice and cultured in the presence of the macrophage colony-stimulating factor (M-CSF) . After seven days of culture, IL-4 was added for 4 and 18 hours.
Keywords: Mouse strain comparision; Gene expression profiling
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| Overall design |
IL-4 induced gene expression was investigated in mouse bone marrow-derived macrophages (BMM) of Irf4-deficient or heterozygous mice. BMM were incubated with mouse recombinant IL-4 for 4 or 18 hours or without for 18 hours. Three independent experiments were performed at each time point (mock, 4 and 18 hours) using littermates for each experiment.
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| Contributor(s) |
Chartouni CE, Rehli M |
| Citation(s) |
20580461 |
| Submission date |
May 14, 2010 |
| Last update date |
May 10, 2018 |
| Contact name |
Michael Rehli |
| E-mail(s) |
michael.rehli@klinik.uni-r.de
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| Organization name |
University Hospital Regensburg
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| Department |
Internal Med III
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| Street address |
F.-J.-Strauss-Allee 11
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| City |
Regensburg |
| ZIP/Postal code |
93042 |
| Country |
Germany |
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| Platforms (1) |
| GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA127069 |
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