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Series GSE218871 Query DataSets for GSE218871
Status Public on Dec 01, 2022
Title PARP1 gene knockout suppresses expression of DNA Base Excision Repair genes
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary PARP1 is a member of various DNA repair pathways - main target for anti-cancer treatment.  We evaluated the effect of topotecan and TDP1 Inhibitor in HEK293 wild-type and PARP1 knockout cells on the gene expression
 
Overall design Knockout HEK293A cell line was generated using the CRISPR/Cas9 method. gRNA were design against region with constitutive protein-encoding exons of Parp1 gene. Then, we performed comparative gene expression profiling analysis of RNA-seq data for HEK293 wild type cells and Parp1 KO under influnce of topotecan, OL9-119 and its combination.
 
Contributor(s) Dyrkheeva NS, Malakhova AA, Zakharenko AL, Okorokova LS, Shtokalo DN, Pavlova SV, Medvedev SP, Zakian SM, Nushtaeva AA, Kabilov MR, Tupikin AA, Luzina OA, Salakhutdinov NF, Lavrik OI
Citation(s) 36982223
Submission date Nov 28, 2022
Last update date Oct 23, 2023
Contact name Maxim Ri
E-mail(s) maxim.ree@gmail.com
Organization name LLC AcademGene
Street address Lavrenteva st. 6
City Novosibirsk
ZIP/Postal code 630090
Country Russia
 
Platforms (1)
GPL30209 MGISEQ-2000RS (Homo sapiens)
Samples (40)
GSM6758017 L01_1, WT, no, biol rep 1
GSM6758018 L01_2, WT, DMSO, biol rep 1
GSM6758019 L01_3, WT, TPC_OL9, biol rep 1
Relations
BioProject PRJNA906061

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE218871_raw_counts.tsv.gz 1.6 Mb (ftp)(http) TSV
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Raw data are available in SRA
Processed data are available on Series record

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