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Series GSE21984 Query DataSets for GSE21984
Status Public on May 01, 2019
Title The LKB1/AMPK pathway is a central mediator of the hepatic xenobiotic response
Organism Mus musculus
Experiment type Expression profiling by array
Summary The serine/threonine kinase LKB1 is a tumor suppressor gene which also plays key roles in metabolic function in peripheral tissues through its direct phosphorylation and activation of the AMP-activated protein kinase (AMPK). The LKB1/AMPK pathway plays key roles in the liver in suppressing transcriptional programs of gluconeogenesis and lipogenesis, and hepatic LKB1 is required for the ability of the type 2 diabetes agent metformin to lower blood glucose levels in mice. To more broadly define how the LKB1/AMPK pathway controls hepatic metabolism, transcriptional profiling was employed using mice with an inducible liver-specific deletion of Lkb1. Unexpectedly, LKB1/AMPK signaling broadly controls the expression of many phase I xenobiotic metabolism genes, including several members of the cytochrome P450 family. In particular, expression of CYP2E1, an important mediator of drug detoxification, was markedly reduced upon LKB1 loss. LKB1 liver-specific knockout mice exposed to hepatocarcinogens, exhibited marked resistance to carcinogen-induced hepatocyte apoptosis, proliferation, senescence, and liver fibrosis and tumorigenesis.
Overall design Total RNA obtained from livers of wild-type and LKB liver-specific knockout mice treated with CCL4, AICAR, or control mineral oil or saline.
Contributor(s) Kohnz RA, Shaw RJ, Yu RT
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Submission date May 25, 2010
Last update date May 01, 2019
Contact name Ruth T Yu
Phone 858-453-4100
Organization name Salk Institute
Street address 10010 N. Torrey Pines Rd.
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (16)
GSM546670 wild-type mice treated with mineral oil_A
GSM546671 wild-type mice treated with mineral oil_B
GSM546672 LKB liver-specific knockout mice treated with mineral oil_A
BioProject PRJNA127197

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Supplementary file Size Download File type/resource
GSE21984_RAW.tar 3.1 Mb (http)(custom) TAR
GSE21984_non-normalized_data.txt.gz 2.5 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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