GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE2205 Query DataSets for GSE2205
Status Public on Apr 01, 2005
Title Analysis of Germ Stem Cell loss in a murine model of ERM deficiency
Organism Mus musculus
Experiment type Expression profiling by array
Summary ERM knockout mice were produced. Testis develop a Sertoli cell only
syndrome, which appears complete by 10 weeks of age in male ERM knockout mice.

Three sets of Affymetrix comparisons were made.
#1 and #2, Wt or ERM knockout testis from 4 week old males, totaly RNA.
This time preceeds any significant histologic difference between the wt or
knockout. The results was the finding that only a very specific set or
markers for spermatogonial STEM CELLs were found to be greatly reduced in the ERM knockout.

#3 and #4. The sertoli cell line TM4 was transduced with a retroviral
ERM. The wild type TM4 cell line was compared to this TM$-ERM cell line,
with the finding that only a few genes were increased in the TM4-ERM line.

#5, #6 and #7. Primary Sertoli cells were purified from 4 week old wild
type or ERM knockout or from 10 week old ERM knockout mice and compared.
Keywords: other
Contributor(s) Chen C, Murphy KM
Citation(s) 16107850
Submission date Jan 28, 2005
Last update date Feb 11, 2019
Contact name Kenneth Martin Murphy
Phone 314 3622009
Organization name Washington University School of Medicine
Department Pathology and Immunology
Lab Murphy Lab
Street address 660 S. Euclid
City St. Louis
State/province MO
ZIP/Postal code 63110
Country USA
Platforms (2)
GPL81 [MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (7)
GSM40125 4 week old Wild type testis 1
GSM40126 4 week old ERM knockout testis
GSM40127 TM4 cell line without transfection of ERM
BioProject PRJNA91215

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary data files not provided

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap