|
| Status |
Public on Apr 01, 2005 |
| Title |
Analysis of Germ Stem Cell loss in a murine model of ERM deficiency |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
ERM knockout mice were produced. Testis develop a Sertoli cell only
syndrome, which appears complete by 10 weeks of age in male ERM knockout mice.
Three sets of Affymetrix comparisons were made.
#1 and #2, Wt or ERM knockout testis from 4 week old males, totaly RNA.
This time preceeds any significant histologic difference between the wt or
knockout. The results was the finding that only a very specific set or
markers for spermatogonial STEM CELLs were found to be greatly reduced in the ERM knockout.
#3 and #4. The sertoli cell line TM4 was transduced with a retroviral
ERM. The wild type TM4 cell line was compared to this TM$-ERM cell line,
with the finding that only a few genes were increased in the TM4-ERM line.
#5, #6 and #7. Primary Sertoli cells were purified from 4 week old wild
type or ERM knockout or from 10 week old ERM knockout mice and compared.
Keywords: other
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| Contributor(s) |
Chen C, Murphy KM |
| Citation(s) |
16107850 |
| Submission date |
Jan 28, 2005 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Kenneth Martin Murphy |
| E-mail(s) |
murphy@pathbox.wustl.edu
|
| Phone |
314 3622009
|
| Organization name |
Washington University School of Medicine
|
| Department |
Pathology and Immunology
|
| Lab |
Murphy Lab
|
| Street address |
660 S. Euclid
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL81 |
[MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (7)
|
| GSM40125 |
4 week old Wild type testis 1 |
| GSM40126 |
4 week old ERM knockout testis |
| GSM40127 |
TM4 cell line without transfection of ERM |
| GSM40128 |
TM4 cell line with transfection of ERM |
| GSM40129 |
Primary Mouse wild type 4 week old Sertoli Cells |
| GSM40130 |
Primary Mouse ERM knockout 4 week old Sertoli Cells |
| GSM40139 |
Primary Mouse ERM knockout 10 week old Sertoli Cells |
|
| Relations |
| BioProject |
PRJNA91215 |
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