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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Sep 19, 2011 |
| Title |
Expression data from quadriceps muscle of WT and ERRgamma transgenic mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We show that the orphan nuclear receptor ERRg is expressed at high levels in type I muscle and when transgenically expressed in anaerobic type II muscles (ERRGO mice) or cultured cells, powerfully regulates VEGF expression, angiogenesis and vascular supply in absence of exercise. ERRGO mice show increased expression of genes promoting fat metabolism, mitochondrial respiration and type I fiber specification. In parallel, the type II muscle in ERRGO mice display an activated angiogenic program marked by myofibrillar induction and secretion of pro-angiogenic factors, frank neo-vascularization and a 100% increase in running endurance. Surprisingly, the induction of VEGF and type I muscle properties by ERRg does not involve the transcriptional co-activator PGC1a. Instead, ERRg genetically activates the energy sensor AMPK which is typically inactive in absence of exercise. Therefore, ERRg and AMPK, known regulators of mitochondrial function and metabolism, together control a novel angiogenic pathway that anatomically synchronizes vascular arborization to oxidative metabolism revealing an exercise-independent mechanism for matching supply and demand.
Keywords: ERRgamma overexpression compared to wild-type
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| Overall design |
Comparison of gene expression from quadriceps muscles isolated from wild type and alpha-skeletal actin-ERRgamma-transgenic mice.
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| Contributor(s) |
Yu RT, Narkar VA, Evans RM |
| Citation(s) |
21356518 |
| Submission date |
Jun 02, 2010 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Ruth T Yu |
| Organization name |
Salk Institute
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| Department |
Gene Expression Lab
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| Lab |
Ronald Evans
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| Street address |
10010 N Torrey Pines Rd
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| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (6)
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| GSM549072 |
wild-type quadriceps, biological rep1 |
| GSM549073 |
wild-type quadriceps, biological rep2 |
| GSM549074 |
wild-type quadriceps, biological rep3 |
| GSM549075 |
ERRgamma transgenic quadriceps, biological rep1 |
| GSM549076 |
ERRgamma transgenic quadriceps, biological rep2 |
| GSM549077 |
ERRgamma transgenic quadriceps, biological rep3 |
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| Relations |
| BioProject |
PRJNA128935 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE22086_RAW.tar |
23.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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