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Series GSE22102 Query DataSets for GSE22102
Status Public on Mar 02, 2012
Title Histone H3 lysine 9 di-methylation as an epigenetic signature of the interferon response (sequencing)
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Effective anti-viral immunity depends on the ability of infected cells or cells triggered with virus-derived nucleic acids to produce type I interferon (IFN), which activates transcription of numerous antiviral genes. However, disproportionately strong or chronic IFN expression is a common cause of inflammatory and autoimmune diseases. Here we describe an epigenetic mechanism that determines cell-type specific differences in IFN and IFN-stimulated gene expression in response to exogenous signals. We identify di-methylation of histone H3 at lysine 9 (H3K9me2) as a suppressor of IFN and IFN-inducible antiviral gene expression. We show that levels of H3K9me2 at IFN and IFN stimulated genes (ISG) correlate inversely with the scope and amplitude of IFN and ISG expression in fibroblasts and dendritic cells. Accordingly, genetic ablation or pharmacological inactivation of lysine methyltransferase G9a, which is essential for the generation of H3K9me2, resulted in phenotypic conversion of fibroblasts into highly potent IFN-producing cells and rendered these cells resistant to pathogenic RNA viruses. In summary, our studies implicate H3K9me2 and enzymes controlling its abundance as key regulators of innate antiviral immunity.
 
Overall design Examination of epigenetic modifications in WT and G9a deficient MEFs and splenic DCs
 
Contributor(s) Fang TC, Tarakhovsky A
Citation(s) 22412156
Submission date Jun 02, 2010
Last update date May 15, 2019
Contact name Terry Fang
E-mail(s) tfang@rockefeller.edu
Phone 212-327-8265
Fax 212-327-8258
Organization name Rockefeller University
Street address 1230 York Ave
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platforms (2)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (48)
GSM549563 H3K9me2 WT 1
GSM549564 H3K9me2 G9a KO 1
GSM549565 H3K9me2 WT 2
This SubSeries is part of SuperSeries:
GSE24826 Histone H3 lysine 9 di-methylation as an epigenetic signature of the interferon response
Relations
SRA SRP002587
BioProject PRJNA133077

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE22102_RAW.tar 1.5 Gb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

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