|
| Status |
Public on May 17, 2011 |
| Title |
Genome-wide gene expression analysis during endotoxin tolerance in PBMCs. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Genome-wide gene expression was measured in peripheral blood mononuclear cells (PBMC) from healthy humans donors, four hours post stimulation of a single or double treatment of LPS and compared to untreated control PBMCs. We show that a second treatment with LPS induces endotoxin tolerance, with a transcription profile similar to that seen during alternative polarization (M2) of mononuclear cells. Microarray processing was performed by the Genome BC Microarray Platform.
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| |
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| Overall design |
Total RNA obtained from PBMCs from blood from healthy humans after single treatment with LPS, two treatments with LPS, or untreated. Comparison of LPS or LPS/LPS to untreated cells shows the gene expression pattern of endotoxin tolerance.
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| |
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| Contributor(s) |
Pena O, Fjell C, Hancock R |
| Citation(s) |
21576504, 25685830 |
| Submission date |
Jun 09, 2010 |
| Last update date |
Mar 20, 2017 |
| Contact name |
Christopher Fjell |
| E-mail(s) |
cfjell@mail.ubc.ca
|
| Organization name |
University of British Columbia
|
| Department |
Medicine
|
| Street address |
Room 166 - 1081 Burrard Street
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V6Z 1Y6 |
| Country |
Canada |
| |
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| Platforms (1) |
| GPL6883 |
Illumina HumanRef-8 v3.0 expression beadchip |
|
| Samples (12)
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|
| Relations |
| BioProject |
PRJNA128643 |
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