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| Status |
Public on Jul 10, 2010 |
| Title |
Differential Transcriptional Changes in Mice Exposed to Chemically Distinct Diesel Samples |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Epidemiological studies have linked exposure to ambient particulate matter (PM) with increased asthmatic symptoms. Diesel exhaust particles (DEP) are a predominant source of vehicle derived ambient PM, and experimental studies have demonstrated that they may have adjuvant potential when given with an antigen. We previously compared 3 DEP samples: N-DEP, A-DEP, and C-DEP in a murine ovalbumin (OVA) mucosal sensitization model and reported the adjuvant activity to be: C-DEP ≈ A-DEP > N-DEP. The present study analyzed gene expression changes from the lungs of these mice. Transcription profiling demonstrated that all the DEP samples altered cytokine and toll-like receptor pathways regardless of type, with or without antigen sensitization. Further analysis of DEP exposure with OVA showed that all DEP treatments altered networks involved in immune and inflammatory responses. The A- and C-DEP/OVA treatments induced differential expression of apoptosis pathways in association with stronger adjuvant responses, while expression of cell cycle control and DNA damage pathways were also altered in the C-DEP/OVA treatment. This comprehensive approach using gene expression analysis to examine changes at a pathway level provides detailed information on events occurring in the lung after DEP exposure, and confirms that the most bioactive sample induced many more individual genes and changes in immuno-regulatory and homeostatic pathways.
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| Overall design |
Female BALB/C mice (8-10 weeks old) were randomly divided into 8 treatment groups containing 3 mice each and exposed to saline, 20 ug ovalbumin, 150 ug diesel exhaust particles (either C-DEP, A-DEP, or N-DEP), or diesel exhaust particles + ovalbumin by intranasal instillation on Days 0 and 13 and necropsied 18 hrs latter.
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| Contributor(s) |
Stevens T, Hester S, Gilmour MI |
| Citation(s) |
27458330 |
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Differential Transcriptional Changes in Mice Exposed to Chemically Distinct Diesel Samples. Tina Stevens, Susan Hester and M. Ian Gilmour Biomedical Informatics Insights 2010:3 29-52.
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| Submission date |
Jun 15, 2010 |
| Last update date |
Jun 27, 2019 |
| Contact name |
Tina Stevens |
| Organization name |
UNC
|
| Street address |
138 marlowe ct
|
| City |
carrboro |
| State/province |
NC |
| ZIP/Postal code |
27510 |
| Country |
USA |
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| Platforms (1) |
| GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA127665 |
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