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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Oct 10, 2010 |
| Title |
Hdac3 is essential for the maintenance of chromatin structure and genome stability |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Deletion of Hdac3 impaired DNA repair and greatly reduced chromatin compaction and heterochromatin content. Liver-specific deletion of Hdac3 culminated in hepatocellular carcinoma. The loss of genomic stability and the impaired response to DNA damage suggested that a high mutation rate stimulated the development of HCC. To begin to assess what pathways were involved in the formation of HCC, we performed gene expression analysis using cDNA microarrys.
In the array data, we noted the enhanced expression of c-Myc, a commonly over expressed oncogene and signatures consistent with activation of the Ras pathway and impairment of the p53 pathway were also identified in this analysis.
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| Overall design |
We isolated total RNA from livers obtained from ~10 month old control (Hdac3FL/+; AlbCre+) or null (Hdac3FL/-; AlbCre+) male mice.
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| Contributor(s) |
Bhaskara S, Knutson SK, Jiang G, Chandrasekharan MB, Wilson AJ, Zheng S, Yenamandra A, Locke K, Yuan J, Bonine-Summers AR, Wells CE, Kaiser JF, Washington KM, Zhao Z, Wagner FF, Sun Z, Xia F, Holson EB, Khabele D, Hiebert SW |
| Citation(s) |
21075309 |
| Submission date |
Jun 18, 2010 |
| Last update date |
Mar 04, 2019 |
| Contact name |
srividya bhaskara |
| E-mail(s) |
srividya.bhaskara@vanderbilt.edu
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| Phone |
615-936-3584
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| Organization name |
Vanderbilt University
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| Department |
Biochemistry
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| Street address |
PRB room 512
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| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37232-6838 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (5)
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| GSM557851 |
Control liver, normal tissue, biological rep1 |
| GSM557852 |
Control liver, normal tissue, biological rep2 |
| GSM557853 |
Null liver, tumor tissue, biological rep1 |
| GSM557854 |
Null liver, tumor tissue, biological rep2 |
| GSM557855 |
Null liver, tumor tissue, biological rep3 |
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| Relations |
| BioProject |
PRJNA128611 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE22457_RAW.tar |
19.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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