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| Status |
Public on Jun 24, 2010 |
| Title |
Gene expression pattern of alveolar macrophages of allergic asthmatics in comparison with control subjects |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The implication of alveolar macrophages (AM) in asthma, a Th2 disease, has not been well characterized. Thus, the goal of this study is to better characterize AM phenotype of allergic asthmatic compared with normal subjects using genomic expression analyses. Microarray analyses were performed with AM isolated from bronchoalveolar lavage. Robust multiarray analysis (RMA) normalization and Smyth’s moderated t test were used to select differentially expressed genes. Fifty differentially expressed genes were identified. Nineteen have been classified in categories linked to stress or immune responses and among them; nine are part of the heat shock protein (HSP) family. Difference of expression for three (HSPD1, PRNP, SERPINH1) of the five selected genes were validated using real-time reverse transcription–polymerase chain reaction. Enzyme linked immunosorbent assay was used to measure the protein level of heat shock protein 60 (HSP60), the protein encoded by HSPD1, and showed difference in AM protein level between allergic asthmatic and control subjects. In summary, this study suggests that HSP gene family, particularly HSP60, is involved in AM functions in a context of allergic asthma. These results also support the involvement of AM immune functions in the development of an allergic asthmatic response.
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| Overall design |
Ten alveolar macrophage samples from bronchoalveolar lavages have been studied. Five of them are from allergic asthmatic subjects and five from control subjects (without allergy and asthma).
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| Contributor(s) |
Laprise C, Madore A, Bissonnette AY, Laviolette M, Perron S, Turmel V |
| Citation(s) |
19913588 |
| Submission date |
Jun 23, 2010 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Catherine Laprise |
| Organization name |
Université du Québec à Chicoutimi
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| Street address |
555 Boul. Université
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| City |
Chicoutimi |
| State/province |
Qc |
| ZIP/Postal code |
G7H 2B1 |
| Country |
Canada |
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| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA128601 |
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