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| Status |
Public on Jun 24, 2010 |
| Title |
Gene expression changes in response to doxycyline-induced expression of proinsulin (C96Y)-GFP in an INS-1 insulinoma cell line |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
|
| Summary |
A doxycyline-inducible INS-1 insulinoma cell line expressing proinsulin (C96Y)-GFP was engineered. Addition of doxycyline causes the production of the proinsulin (C96Y)-GFP, which is retained in the endoplasmic reticulum. This study analyzes the gene expression changes that occur after doxycyline-induced expression of proinsulin (C96Y)-GFP for 24h, 48h and 5 days. Expression changes were compared between control un-induced cells and cells treated with doxycyline. Three replicates (experiments) were performed for each time point.
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| |
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| Overall design |
Stable mutant insulin-expressing insulinoma cells were treated or not with doxycyline for 24h, 48h or 5 days and RNA was extracted for expression analysis.
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| |
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| Contributor(s) |
Volchuk A |
| Citation(s) |
20659334 |
| Submission date |
Jun 23, 2010 |
| Last update date |
Jul 31, 2017 |
| Contact name |
Allen Volchuk |
| E-mail(s) |
avolchuk@uhnres.utoronto.ca
|
| Phone |
416 581 7675
|
| Organization name |
Toronto General Research Inst.
|
| Department |
Cellular & Molecular Biology
|
| Street address |
101 College Street
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 1L7 |
| Country |
Canada |
| |
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| Platforms (1) |
| GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA128457 |
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