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| Status |
Public on Aug 12, 2010 |
| Title |
Control of Embryonic Stem Cell State by Mediator and Cohesin (Agilent gene expression data) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The key transcription factors that control the embryonic stem cell gene expression program have been identified, but how they function to implement this program is not well understood. While screening for genes essential for maintenance of ES cell state, we identified many components of the Mediator and Cohesin complexes. Mediator and Cohesin were found to physically and functionally connect the enhancers and core promoters of active genes. An ES cell Mediator complex was found to copurify with Cohesin and its loading factor Nipbl, and normal levels of these proteins were essential for expression of the genes they occupy and for maintenance of ES cell state.
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| Overall design |
See associated publication.
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| Contributor(s) |
Kagey MH, Bilodeau S, Newman JJ, Orlando DA, Young RA |
| Citation(s) |
20720539 |
| Submission date |
Jun 24, 2010 |
| Last update date |
May 10, 2018 |
| Contact name |
Richard A Young |
| E-mail(s) |
young_computation@wi.mit.edu
|
| Phone |
617-258-5219
|
| Organization name |
Whitehead Institute for Biomedical Research
|
| Lab |
Young Lab
|
| Street address |
9 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
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| Samples (5)
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| This SubSeries is part of SuperSeries: |
| GSE22557 |
Control of Embryonic Stem Cell State by Mediator and Cohesin |
|
| Relations |
| BioProject |
PRJNA129101 |
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