|
| Status |
Public on Jun 29, 2010 |
| Title |
Gene expression profiles of adult glomerular endothelial cells isolated from TIE2-GFP transgenic mice using FACS. (GUDMAP Series ID: 42) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing kidney. The central thesis is straightforward. The combination of fluorescent activated cell sorting (FACS) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing kidney. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene in FACS isolated components of the developing kidney. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone.
|
| |
|
| Overall design |
Tie2-GFP transgenic mice were utilized to isolate the endothelial cell population from the glomerulus of adult kidneys. The glomerular endothelial cells were isolated from kidneys using a combination of collagenase treatment, differential seiving, trypsin treatment and FACS. The RNA was isolated from purified endothelial cells and the gene expression profiles were determined by microarrays.
|
| Web link |
http://www.gudmap.org
|
| |
|
| Contributor(s) |
Potter S, Brunskill E |
| Citation(s) |
20706631 |
| Submission date |
Jun 24, 2010 |
| Last update date |
Mar 04, 2019 |
| Contact name |
GUDMAP Developers |
| E-mail(s) |
gudmap-db@gudmap.org
|
| Phone |
+44 131 651 8500
|
| Organization name |
IGMM MRC Human Genetics Unit
|
| Lab |
GUDMAP Database Group
|
| Street address |
Crewe Road
|
| City |
Edinburgh |
| ZIP/Postal code |
EH4 2XU |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (3) |
|
| Relations |
| BioProject |
PRJNA128445 |
