|
| Status |
Public on Aug 12, 2010 |
| Title |
Control of Embryonic Stem Cell State by Mediator and Cohesin (Illumina ChIP-Seq data) |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The key transcription factors that control the embryonic stem cell gene expression program have been identified, but how they function to implement this program is not well understood. While screening for genes essential for maintenance of ES cell state, we identified many components of the Mediator and Cohesin complexes. Mediator and Cohesin were found to physically and functionally connect the enhancers and core promoters of active genes. An ES cell Mediator complex was found to copurify with Cohesin and its loading factor Nipbl, and normal levels of these proteins were essential for expression of the genes they occupy and for maintenance of ES cell state.
|
| |
|
| Overall design |
See associated publication.
Sample GSM555166 (GSE22320) was used as a negative control.
|
| |
|
| Contributor(s) |
Kagey MH, Bilodeau S, Newman JJ, Orlando DA, Young RA |
| Citation(s) |
20720539 |
| Submission date |
Jun 24, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Richard A Young |
| E-mail(s) |
young_computation@wi.mit.edu
|
| Phone |
617-258-5219
|
| Organization name |
Whitehead Institute for Biomedical Research
|
| Lab |
Young Lab
|
| Street address |
9 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
|
| Samples (17)
|
| GSM560341 |
ChIP-Seq for Smc1 in mES cells |
| GSM560342 |
ChIP-Seq for Smc1 in mES cells (rep 2) |
| GSM560343 |
ChIP-Seq for Smc3 in mES cells (rep 2) |
|
| This SubSeries is part of SuperSeries: |
| GSE22557 |
Control of Embryonic Stem Cell State by Mediator and Cohesin |
|
| Relations |
| SRA |
SRP002778 |
| BioProject |
PRJNA129097 |
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