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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Oct 15, 2010 |
| Title |
Organotpyic Human Epithelial Neoplasia |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Refined cancer models are required to assess the burgeoning number of potential targets for cancer therapeutics within a rapid and clinically relevant context. Here we utilize tumor-associated genetic pathways to transform primary human epithelial cells from epidermis, oropharynx, esophagus, and cervix into genetically defined tumors within an entirely human 3-dimensional (3-D) tissue environment incorporating cell-populated stroma and intact basement membrane (BM). These engineered organotypic tissues recapitulated natural features of tumor progression, including epithelial invasion through the BM, a complex process critically required for biologic malignancy in 90% of human cancers. Invasion was rapid, and potentiated by stromal cells. Oncogenic signals in 3-D tissue, but not 2-D culture, resembled gene expression profiles from spontaneous human cancers. Screening well-characterized signaling pathway inhibitors in 3-D organotypic neoplasia helped distil a clinically faithful cancer gene signature. Multi-tissue 3-D human tissue cancer models may provide an efficient and relevant complement to current approaches to characterize cancer progression.
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| Overall design |
Organotypic human epidermal epithium expressing LacZ, cdk4 and Hras, or cdk and Ras with U0126 mediated MEK inhibition were harvested for RNA extraction and hybridization on Affymetrix microarrays. There are 8 biologic replicates for the LacZ, and cdk4 Ras groups, and 2 biologic replicates for the U0126 treated samples.
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| Contributor(s) |
Ridky TW, Khavari PA |
| Citation(s) |
21102459 |
| Submission date |
Jun 25, 2010 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Todd W. Ridky |
| Organization name |
Stanford University
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| Department |
Dermatology
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| Lab |
Khavari
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| Street address |
269 Campus Drive
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| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (18)
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| GSM560270 |
organotypic epidermis LacZ rep4 |
| GSM560271 |
organotypic epidermis LacZ rep5 |
| GSM560272 |
organotypic epidermis LacZ rep6 |
| GSM560273 |
organotypic epidermis LacZ rep7 |
| GSM560274 |
organotypic epidermis LacZ rep8 |
| GSM560275 |
organotypic epidermis cdk4 Ras rep1 |
| GSM560276 |
organotypic epidermis cdk4 Ras rep |
| GSM560277 |
organotypic epidermis cdk4 Ras rep3 |
| GSM560278 |
organotypic epidermis cdk4 Ras rep4 |
| GSM560279 |
organotypic epidermis cdk4 Ras rep5 |
| GSM560280 |
organotypic epidermis cdk4 Ras rep6 |
| GSM560281 |
organotypic epidermis cdk4 Ras rep7 |
| GSM560282 |
organotypic epidermis cdk4 Ras rep8 |
| GSM560283 |
organotypic epidermis cdk4 Ras U0126 rep1 |
| GSM560284 |
organotypic epidermis cdk4 Ras U0126 rep 2 |
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| Relations |
| BioProject |
PRJNA128421 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE22573_RAW.tar |
35.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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