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| Status |
Public on Apr 01, 2011 |
| Title |
Expression data from Caenorhabditis elegans exposed to ivermectin |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by array
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| Summary |
We have investigated the transcriptomic response of the model nematode Caenorhabditis elegans to ivermectin (IVM); an important anthelmintic for human and animal parasite control.
The transcriptomic response of the mutant strain DA1316 avr-14(ad1302); avr-15(ad1250); glc-1(pk54), which is highly resistant to ivermectin due to null mutations in three glutamate-gated chloride channel subunits, was examined. Despite the resistant nature of this strain, pharyngeal pumping rate was decreased following 4 hrs exposure to 100ng/ml and 1μg/ml ivermectin resulting in significant change in the expression level of genes associated with a fasting response.
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| Overall design |
Matched cultures of synchronised C. elegans were grown to L4 stage on standard NGM plates with an OP50 bacterial lawn. The nematodes were then transferred to NGM plates containing 100ng/ml ivermectin, 1μg/ml ivermectin, or DMSO excipient only (control) for 4 hours. RNA was extracted from five biological replicates including controls for both the 100ng/ml ivermectin and 1μg/ml ivermectin experiments and hybridised to Affymetrix arrays.
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| Contributor(s) |
Laing ST, Ivens A, Gilleard JS |
| Citation(s) |
22348077 |
| Submission date |
Jul 01, 2010 |
| Last update date |
Mar 09, 2021 |
| Contact name |
Steven Thomas Laing |
| E-mail(s) |
steven.t.laing@googlemail.com
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| Organization name |
University of Glasgow
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| Department |
Veterinary Medicine
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| Lab |
Gilleard lab (Henry Wellcome Building)
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| Street address |
464 Bearsden Road
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| City |
Glasgow |
| ZIP/Postal code |
G61 1QH |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL200 |
[Celegans] Affymetrix C. elegans Genome Array |
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| Samples (20)
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| GSM562065 |
100ng/ml ivermectin exposure, biological rep 1 |
| GSM562066 |
100ng/ml ivermectin exposure, biological rep 2 |
| GSM562067 |
100ng/ml ivermectin exposure, biological rep 3 |
| GSM562068 |
100ng/ml ivermectin exposure, biological rep 4 |
| GSM562069 |
100ng/ml ivermectin exposure, biological rep 5 |
| GSM562070 |
100ng/ml DMSO control, biological rep 1 |
| GSM562071 |
100ng/ml DMSO control, biological rep 2 |
| GSM562072 |
100ng/ml DMSO control, biological rep 3 |
| GSM562073 |
100ng/ml DMSO control, biological rep 4 |
| GSM562074 |
100ng/ml DMSO control, biological rep 5 |
| GSM562075 |
1ug/ml ivermectin exposure, biological rep 1 |
| GSM562076 |
1ug/ml ivermectin exposure, biological rep 2 |
| GSM562077 |
1ug/ml ivermectin exposure, biological rep 3 |
| GSM562078 |
1ug/ml ivermectin exposure, biological rep 4 |
| GSM562079 |
1ug/ml ivermectin exposure, biological rep 5 |
| GSM562080 |
1ug/ml DMSO control, biological rep 1 |
| GSM562081 |
1ug/ml DMSO control, biological rep 2 |
| GSM562082 |
1ug/ml DMSO control, biological rep 3 |
| GSM562083 |
1ug/ml DMSO control, biological rep 4 |
| GSM562084 |
1ug/ml DMSO control, biological rep 5 |
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| Relations |
| BioProject |
PRJNA128009 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE22660_RAW.tar |
40.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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