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| Status |
Public on Oct 20, 2010 |
| Title |
Genome-wide mapping of PRDM14 binding sites in human embryonic stem cells |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
PRDM14 belongs to the PR (PRDI-BF1 and RIZ) domain proteins (PRDM) family which is a subclass of the SET domain proteins, a common domain found in histone modifying enzymes. PRDM14 has been previously implicated to regulate self-renewal of hESCs as knock-down of PRDM14 induced expression of differentiation marker genes and altered the cellular morphology. We showed that PRDM14 directly regulates the expression of key pluripotency gene POU5F1. Genome-wide location profiling experiments revealed that PRDM14 co-localized extensively with other key transcription factors such as OCT4, NANOG and SOX2. More importantly, in a gain-of-function assay, we showed that PRDM14 is able to enhance the efficiency of reprogramming of human fibroblasts in conjunction with OCT4, SOX2 and KLF4. Hence, PRDM14 exemplifies a key transcription factor that is required for the maintenance of human ESC identity and the reacquisition of pluripotency in human somatic cells.
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| |
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| Overall design |
ChIP-seq of PRDM14 in human ESCs
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| |
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| Contributor(s) |
Ng H, Chan Y, Chia N, Orlov YL |
| Citation(s) |
20953172 |
| Submission date |
Jul 06, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Yuriy L Orlov |
| E-mail(s) |
orlovy@gis.a-star.edu.sg
|
| Organization name |
Genome Institute of Singapore
|
| Department |
Computational Systems Biology
|
| Street address |
60 Biopolis Street
|
| City |
Singapore |
| ZIP/Postal code |
138672 |
| Country |
Singapore |
| |
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| Platforms (1) |
| GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
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| Samples (2) |
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| Relations |
| SRA |
SRP002814 |
| BioProject |
PRJNA128257 |
