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| Status |
Public on Jul 07, 2010 |
| Title |
Pbx1-d Induces T cell Activation and a Decreased Apoptosis in Response to Retinoic Acids (RA) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The hypothesis was tested that the Pbx1-d isoform was responsible for the Sle1a.1 phenotypes in CD4+ T cells.
Jurkat T cells were transfected with a lentiviral construct expressing Pbx1-d-GFP or control RFP. Pbx1-d over-expression reduced the percentage of late apoptotic cells in response to anti-CD3 and anti-CD28 stimulation as compared with control-Lin28-transfected cells. Overall, these data demonstrate that over-expression of Pbx1-d results in an activated/inflammatory phenotype and in a defective response to RA in Jurkat T cells, strongly suggesting that the increased expression of Pbx1-d is responsible for the Sle1a.1 phenotypes.
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| Overall design |
Jurkat T cells (5 x 105 cells/ml) were transfected with an lentiviral (LV) vector expressing either control (RFP or Lin28) or Pbx1-d-GFP. Total RNA was extracted from GFP+ or RFP+ FACS-sorted Jurkat T cells transfected LV-GFP-Pbx1-d or LV-RFP, as well as non-transfected cells in 4 independent transfections per group.
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| Contributor(s) |
Cuda CM, Li S, Potula HH, Xu Z, Butfiloski E, Chang L, Dozmorov I, Sobel ES, Morel L |
| Citation(s) |
22180614 |
| Submission date |
Jul 07, 2010 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Igor M Dozmorov |
| E-mail(s) |
igor-dozmorov@omrf.org
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| Phone |
405-271-7052
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| Fax |
405-271-7063
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| URL |
http://omrf.org
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| Organization name |
OMRF
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| Department |
Arthritis & Immunology
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| Street address |
825 NE 13th Street
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| City |
Oklahoma City |
| State/province |
OK |
| ZIP/Postal code |
73162 |
| Country |
USA |
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| Platforms (1) |
| GPL6884 |
Illumina HumanWG-6 v3.0 expression beadchip |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA127921 |
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