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Series GSE22856 Query DataSets for GSE22856
Status Public on Jul 09, 2011
Title Expression data of lung tissues from ICR mice treated with or without single-walled carbon nanotubes (SWCNTs)
Organism Mus musculus
Experiment type Expression profiling by array
Summary The SWCNTs can induce airway hyper-responsiveness (AHR) and chronic obstructive pulmonary disease (COPD)-like changes in mice model. The SWCNTs can induce clusters of proteinases, chemokines/cytokines, and macrophage receptors triggered signaling, which might play critical roles in the SWCNTs-induced pathological changes, including chronic inflammation and tissues remodeling.
We used microarrays to detail the global programme of gene expression underlying and identified distinct classes of up-regulated genes during this process.
 
Overall design Lung tissues from adult ICR mice (6 weeks old) treated with or without single dose of 0.5 mg SWCNTs by intratrachael instillation were collected and homogenated under liquid nitrogen for RNA extraction and hybridization on Affymetrix microarrays. Since our data showed that the SWCNTs could induce significantly pulmonary granuloma and COPD-like lesion. Two time points of the samples have been collected for microarray analysis: day 1 after SWCNTs treatment as the acute phase responses; day 7 as the sub-chronic responses. We tried to compare the SWCNTs-regulated gene expression profiles with the PF68-treated (as the solvent control) to identify the possible mechanisms of SWCNTs-induced toxicity in lung.
 
Contributor(s) Hsieh W, Chou C, Ho C, Yu S, Chen H, Chou HE, Chen JJ, Chen H, Yang P
Citation(s) 21960547
Submission date Jul 09, 2010
Last update date Feb 11, 2019
Contact name Huei-Wen Chen
E-mail(s) shwchen@ntu.edu.tw
Phone +886-2-23123456
Fax +886-2-23958341
Organization name National Taiwan University
Department Medical College
Lab Graduate Institute of Toxicology
Street address No.1, Sec.1, Re-Ai Rd
City Taipei
ZIP/Postal code 100
Country Taiwan
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (8)
GSM564815 lung tissues PF68 treated on day 1, biological rep1
GSM564816 lung tissues PF68 treated on day 1, biological rep2
GSM564817 lung tissues 0.5mg SWCNTs treated on day 1, biological rep1
Relations
BioProject PRJNA128065

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE22856_RAW.tar 28.3 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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