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Status |
Public on Jul 05, 2011 |
Title |
FTO: gene expression and function in skeletal muscle |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
We identified the target genes of FTO ("fat mass and obesity associated") in primary cultures of human skeletal muscle cells using adenoviral vectors expressing FTO or GFP and oligonucleotide microarrays.
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Overall design |
Human myotubes were prepared from 4 different skeletal muscle biopsies. After differentiation, myotubes were infected for 48 hours with recombinant adenovirus expressing either GFP or FTO. Each FTO-infected myotubes culture was compared to GFP-infected myotubes culture. GFP-infected myotubes were regarded as the control. Four biological replicates were processed.
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Contributor(s) |
Bravard A, Pesenti S, Meugnier E, Rieusset J |
Citation(s) |
20943749 |
Submission date |
Jul 09, 2010 |
Last update date |
Jan 18, 2013 |
Contact name |
emmanuelle meugnier |
E-mail(s) |
meugnier@univ-lyon1.fr
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Organization name |
INSERMU1060/INRA1397
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Lab |
CarMeN
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Street address |
Cens Eli Bat 2 D- Hopital Lyon Sud
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City |
Pierre BĂ©nite |
ZIP/Postal code |
69495 |
Country |
France |
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Platforms (1) |
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Samples (4)
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Relations |
BioProject |
PRJNA128067 |