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Series GSE22862 Query DataSets for GSE22862
Status Public on Apr 08, 2011
Title Prognostic Gene Expression Signature of Carcinoma Associated Fibroblasts in Non-Small Cell Lung Cancer [expression profiling_CAFs]
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The tumor microenvironment strongly influences cancer development, progression and metastasis. The role of carcinoma-associated fibroblasts (CAFs) in these processes and their clinical impact has not been studied systematically in non-small cell lung carcinoma (NSCLC). We established primary cultures of CAFs and matched normal fibroblasts (NFs) from 15 resected NSCLC. We demonstrate that CAFs have greater ability than NFs to enhance the tumorigenicity of lung cancer cell lines. Microarray gene expression analysis of the 15 matched CAF and NF cell lines identified 46 differentially expressed genes, encoding for proteins that are significantly enriched for extracellular proteins regulated by the TGF-beta signaling pathway. We have identified a subset of 11 genes that formed a prognostic gene expression signature, which was validated in multiple independent NSCLC microarray datasets. Functional annotation using protein-protein interaction analyses of these and published cancer stroma-associated gene expression changes revealed prominent involvement of the focal adhesion and MAPK signalling pathways. Fourteen (30%) of the 46 genes also were differentially expressed in laser-capture micro-dissected corresponding primary tumor stroma compared to the matched normal lung. Six of these 14 genes could be induced by TGF-beta1 in NF. The results establish the prognostic impact of CAF-associated gene expression changes in NSCLC patients.
 
Overall design 30 samples: 15 primary NSCLC fibroblast cell lines, 15 normal lung fibroblast cell lines
 
Contributor(s) Navab R, Strumpf D, Tsao M
Citation(s) 21474781
Submission date Jul 09, 2010
Last update date Feb 18, 2019
Contact name Ming-Sound Tsao
E-mail(s) Ming.Tsao@uhn.on.ca
Phone (416) 946-4426
Organization name Ontario Cancer Institute/ Princess Margaret Hospital
Street address
City Toronto
ZIP/Postal code M5G 2M9
Country Canada
 
Platforms (1)
GPL5175 [HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [transcript (gene) version]
Samples (30)
GSM564890 primary_NSCLC_fibroblast_cell_line520
GSM564891 primary_NSCLC_fibroblast_cell_line746
GSM564892 primary_NSCLC_fibroblast_cell_line971
This SubSeries is part of SuperSeries:
GSE22874 Prognostic Gene Expression Signature of Carcinoma Associated Fibroblasts in Non-Small Cell Lung Cancer
Relations
BioProject PRJNA129137

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE22862_RAW.tar 627.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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