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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 08, 2012 |
| Title |
Profiling of direct mRNA targets of miR-130a, miR-203 and miR-205 in prostate cancer cell line LNCaP |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Micro RNAs (miRNAs) miR-130a, miR-203 and miR-205 are jointly downregulated in prostate cancer and act as repressors of AR-signaling. MiRNAs are small non-coding RNAs that regulate the expression of specific mRNA targets mainly by translational repression, mRNA deadenylation or cleavage. Reconstitution of these lost miRNAs in the LNCaP PCa cell line cause morphology changes, growth arrest, and apoptosis, increasing when the miRNAs were co-expressed. This series identifies direct targets of miR-130a, miR-203, and miR-205 by AGO2-RNA co-immunoprecipitation as described by (Beitzinger et al. 2007) upon miRNA reconstitution in LNCaP cells and analyzing AGO2-bound mRNAs using Affymetrix Genechips. Relative levels of AGO2 bound versus total RNA expression were compared between miRNA reconstituted and miR-scr transfected samples.
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| Overall design |
Three arrays each for AGO2-bound RNA upon reconstitution of miR-130a, miR-203, miR-205, a scramble miRNA, and three arrays each for total RNA upon reconstitution of miR-130a, miR-203, miR-205, a scramble miRNA.
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| Contributor(s) |
Boll K, Reiche K, Mörbt N, Kretzschmar AK, Schutt K, Tomm J, Verhaegh G, Schalken J, von Bergen M, Horn F, Hackermüller J |
| Citation(s) |
22391564 |
| Submission date |
Jul 16, 2010 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Kristin Reiche |
| E-mail(s) |
kristin.reiche@izi.fraunhofer.de
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| Organization name |
Fraunhofer Institute for Cell Therapy and Immunology
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| Department |
Diagnostics
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| Street address |
Perlickstr. 1
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| City |
Leipzig |
| ZIP/Postal code |
04103 |
| Country |
Germany |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (24)
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| GSM567159 |
AGO2-RNA upon miR-130a reconstitution in LNCaP, biological replicate 1 |
| GSM567160 |
AGO2-RNA upon miR-130a reconstitution in LNCaP, biological replicate 2 |
| GSM567161 |
AGO2-RNA upon miR-130a reconstitution in LNCaP, biological replicate 3 |
| GSM567162 |
Total RNA upon miR-130a reconstitution in LNCaP, biological replicate 1 |
| GSM567163 |
Total RNA upon miR-130a reconstitution in LNCaP, biological replicate 2 |
| GSM567164 |
Total RNA upon miR-130a reconstitution in LNCaP, biological replicate 3 |
| GSM567165 |
AGO2-RNA upon miR-203 reconstitution in LNCaP, biological replicate 1 |
| GSM567166 |
AGO2-RNA upon miR-203 reconstitution in LNCaP, biological replicate 2 |
| GSM567167 |
AGO2-RNA upon miR-203 reconstitution in LNCaP, biological replicate 3 |
| GSM567168 |
Total RNA upon miR-203 reconstitution in LNCaP, biological replicate 1 |
| GSM567169 |
Total RNA upon miR-203 reconstitution in LNCaP, biological replicate 2 |
| GSM567170 |
Total RNA upon miR-203 reconstitution in LNCaP, biological replicate 3 |
| GSM567171 |
AGO2-RNA upon miR-205 reconstitution in LNCaP, biological replicate 1 |
| GSM567172 |
AGO2-RNA upon miR-205 reconstitution in LNCaP, biological replicate 2 |
| GSM567173 |
AGO2-RNA upon miR-205 reconstitution in LNCaP, biological replicate 3 |
| GSM567174 |
Total RNA upon miR-205 reconstitution in LNCaP, biological replicate 1 |
| GSM567175 |
Total RNA upon miR-205 reconstitution in LNCaP, biological replicate 2 |
| GSM567176 |
Total RNA upon miR-205 reconstitution in LNCaP, biological replicate 3 |
| GSM567177 |
AGO2-RNA upon scramble miR reconstitution in LNCaP, biological replicate 1 |
| GSM567178 |
AGO2-RNA upon scramble miR reconstitution in LNCaP, biological replicate 2 |
| GSM567179 |
AGO2-RNA upon scramble miR reconstitution in LNCaP, biological replicate 3 |
| GSM567180 |
Total RNA upon scramble miR reconstitution in LNCaP, biological replicate 1 |
| GSM567181 |
Total RNA upon scramble miR reconstitution in LNCaP, biological replicate 2 |
| GSM567182 |
Total RNA upon scramble miR reconstitution in LNCaP, biological replicate 3 |
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| This SubSeries is part of SuperSeries: |
| GSE17362 |
miRNA expression, mRNA expression upon miRNA reconstitution, and direct mRNA target identification in prostate cancer cell lines |
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| Relations |
| BioProject |
PRJNA127757 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE22979_RAW.tar |
36.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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