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Series GSE22989 Query DataSets for GSE22989
Status Public on Nov 29, 2011
Title Gene expression profiling of Tgfbr2 mutant mouse models of cleft palate
Organism Mus musculus
Experiment type Expression profiling by array
Summary The overall goal of this project is to investigate the role of TGF-beta signaling in palate development in order to discover candidate therapeutics for preventing and treating congenital birth defects. Here, we conducted gene expression profiling of embryonic palatal tissue from wild type mice as well as those with a neural crest specific conditional inactivation of the Tgfbr2 gene. The latter mice provide a model of cleft palate formation.
 
Overall design To investigate the mechanism of cleft palate resulting from mutations in TGFBR2, we analyzed neural crest specific conditional inactivation of Tgfbr2 in mice (Tgfbr2fl/fl;Wnt1-Cre). We performed microarray analyses using the palatal tissue of Tgfbr2fl/fl;Wnt1-Cre mice at embryonic day E13.5 (prior to palatal fusion, n=6 per genotype) and E14.5 (during palatal fusion, n=5 per genotype) to examine the genes regulated by Tgf-beta during palate formation.
 
Contributor(s) Iwata J, Hacia JG, Chai Y
Citation(s) 22123828
Submission date Jul 19, 2010
Last update date Feb 11, 2019
Contact name Joseph Gerard Hacia
E-mail(s) hacia@hsc.usc.edu
Phone 323-442-3030
Organization name University of Southern California
Department Biochemistry and Molecular Biology
Lab Hacia Lab
Street address 2250 Alcazar Street, IGM 261
City Los Angeles
State/province CA
ZIP/Postal code 90089
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (10)
GSM564967 E14.5 Wnt1-Cre;Tgfbr2 Conditional Knockout Mouse Model Palate 1
GSM564968 E14.5 Wnt1-Cre;Tgfbr2 Conditional Knockout Mouse Model Palate 2
GSM564969 E14.5 Wnt1-Cre;Tgfbr2 Conditional Knockout Mouse Model Palate 3
Relations
BioProject PRJNA127771

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE22989_RAW.tar 37.3 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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