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Series GSE22993 Query DataSets for GSE22993
Status Public on Dec 31, 2013
Title Global gene profiling of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-induced neuronal death
Organism Mus musculus
Experiment type Expression profiling by array
Summary AMPA receptors are involved not only in neuronal plasticity but also in excitotoxicity, mediated largely by the influx of Ca2+ (Choi et al., 1988). Their implication has been highlighted in animal models of ischemia and epilepsy. Studies of ischemic rodent models featured that prior to cell death, hippocampal CA1 pyramidal cells exhibit an increased AMPA receptor-mediated Ca2+ influx and decreased GluR2 and GluR3 mRNA and protein levels (Gorter et al., 1997; Heuerteaux et al., 1995).
 
Overall design A total of 15 RNA samples were analyzed. Cultured murine primary cortical neurons were treated with 300uM AMPA over a time-course of 5h, 15h and 24h (n=3) in addition to the vehicle control (n=6).
 
Contributor(s) Cheung NS, Chen MJ, Peng ZF, Manikandan J
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Submission date Jul 19, 2010
Last update date Dec 31, 2013
Contact name Minghui Jessica Chen
Organization name Menzies Research Institute
Department Neuroscience group
Lab A/P Steve Cheung
Street address Menzies Research Institute, University of Tasmania, Private Bag 24
City Hobart
State/province Tasmania
ZIP/Postal code 7001
Country Australia
 
Platforms (1)
GPL6103 Illumina mouseRef-8 v1.1 expression beadchip
Samples (15)
GSM567487 Cortical neurons-Ctrl-Rep 1 [AMPA, KA]
GSM567488 Cortical neurons-Ctrl-Rep 2 [AMPA, KA]
GSM567489 Cortical neurons-Ctrl-Rep 3 [AMPA, KA]
Relations
BioProject PRJNA127829

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE22993_AMPA_and_KA_Sample_Gene_Profile.txt.gz 6.4 Mb (ftp)(http) TXT
GSE22993_RAW.tar 3.4 Mb (http)(custom) TAR
GSE22993_non_normalized.txt.gz 1.9 Mb (ftp)(http) TXT
GSE22993_normalized.txt.gz 2.6 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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