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| Status |
Public on Jun 02, 2005 |
| Title |
Rat liver response to Clofibrate, DEHP or VPA |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
The project had 2 goals:
1) To evaluate the transcriptional response of 3 prototypical toxicants (Clofibrate, VPA, and DEHP) on rat lever.
2) To evaluate the impact pooling samples has on data analysis.
Keywords = Clofibrate
Keywords = Diethylhexyl phthalate
Keywords = Phthalic acid bis(2-ethylhexyl ester)
Keywords = Sodium Valproate
Keywords = Valproic Acid
Keywords = VPA
Keywords = vehicle
Keywords: other
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| Overall design |
Male SD rats were given a single dose of one of 3 prototypical toxicants or dosed with vehicle. The toxicants used were:
1000mg/kg Clofibrate (in 0.9% saline)
2000mg/kg Sodium Valproate (in distilled water)
20g/kg DEHP (in distilled water)
Animals were sacrificed at 4, 24, 48, or 168hrs post-dosing (only 48 and 168hrs for vehicle-treated animals)
One of the goals of this study was to assess the impact pooling RNA samples has on the array data. For each treatment and vehicle group there are 3-5 individual samples, as well as 1 pooled sample.
10ug of total RNA were hybridized to Affymetrix RG_U34A arrays according to the manufacturer's protocol. Signal intensities were generated using MAS5, with global scaling set to 1500.
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| Contributor(s) |
Jolly RA, Goldstein KM, Wei T, Gao H, Chen P, Huang S, Colet J, Ryan TP, Thomas CE, Estrem ST |
| Citation(s) |
15914576 |
| Submission date |
Feb 22, 2005 |
| Last update date |
Feb 21, 2017 |
| Contact name |
Keith M Goldstein |
| E-mail(s) |
goldsteinkm@lilly.com
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| Organization name |
Eli Lilly & Co.
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| Department |
Investigative Toxicology
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| Lab |
Keith Goldstein
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| Street address |
Lilly Corporate Center
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| City |
Indianapolis |
| State/province |
IN |
| ZIP/Postal code |
46285 |
| Country |
USA |
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| Platforms (1) |
| GPL85 |
[RG_U34A] Affymetrix Rat Genome U34 Array |
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| Samples (94)
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| Relations |
| BioProject |
PRJNA91687 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE2303_RAW.tar |
457.5 Mb |
(http)(custom) |
TAR (of CEL, CHP, EXP) |
| Processed data provided as supplementary file |
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