|
| Status |
Public on Jul 24, 2010 |
| Title |
ChIP-Seq data for histone marks in estrogen-inducible MyoD1 fibroblasts |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The conversion of fibroblasts to myotubes by forced expression of MyoD1 is among the earliest demonstrations of reprogramming of vertebrate cell state. To gain insights into the chromatin state of genes required for reprogramming, we profiled H3K4me3, H3K27me3 and H3K9me3.
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| |
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| Overall design |
DNA was enriched by chromatin immunoprecipitation (ChIP) and analyzed by Solexa sequencing. ChIP was performed using an antibody against H3K4me3, H3K27me3 and H3K9me3.
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| |
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| Contributor(s) |
Bilodeau S, Young RA |
| Citation missing |
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| Submission date |
Jul 22, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Richard A Young |
| E-mail(s) |
young_computation@wi.mit.edu
|
| Phone |
617-258-5219
|
| Organization name |
Whitehead Institute for Biomedical Research
|
| Lab |
Young Lab
|
| Street address |
9 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
|
| Samples (4)
|
| GSM569075 |
ChIP-Seq for H3K27me3 in MDER 10T cells |
| GSM569076 |
ChIP-Seq for H3K4me3 in MDER 10T |
| GSM569077 |
ChIP-Seq for H3K9me3 in MDER 10T cells |
|
| Relations |
| SRA |
SRP003023 |
| BioProject |
PRJNA131689 |
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