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| Status |
Public on Dec 31, 2011 |
| Title |
Cellular response to protein-conjugated nanoparticles |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Interaction of nanomaterials with the mammalian cells remains to be poorly understood at the molecular level. Here we report the first synthesis of hybrid nanomaterial termed phage mimetic nanorods (PMN’s) inspired from filamentous bacteriophage present in nature. We emulate filamentous bacteriophage structure by combining two separate nanoscale entities, one functionalized gold nanorods and the other is coat proteins isolated from filamentous bacteriophage obtained through landscape phage screening. Utilizing biochemical interactions between a phage-derived protein and functionalized gold nanorods, we synthesized novel phage mimetic nanorods. The resulting nanovectors showed excellent multi functional properties including target specificity, imaging and photo destruction ability in a model SKBR-3 breast cancer cells. In addition, to gain insight into the molecular interactions involved during internalization of PMNs by SKBR-3 cells, we performed gene expression profiling of cells exposed to PMNs as compared with naive cells. We identified 70 upregulated and 26 downregulated genes responding to PMNs. Heparin binding internalization was identified as most plausible mechanisms of PMNs entry. Members of Major Histocompatibility complex I (MHC class I) were activated by PMNs, leading to enhanced lysosome and proteasome activity. Seven members of poorly annotated G antigen family (GAGE) of genes were upregulated, and may represent novel mechanism of PMNs entry recognition by cells. In summary, we present a versatile technique of PMNs production as any protein isolated from phage libraries selected against any in vitro and in vivo cells can be used as a source to synthesize PMN’s. This study also opens new avenues to understand the role of nanomaterials at the molecular level.
Investigate the effect of phage mimetic nanorods onto SKBT-3 cells
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| Overall design |
Two groups were compared, three biological replicates each. One group was control cells and the other was test.
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| Contributor(s) |
Abbineni G, Liu A, Dozmorov M, Qui P, Modali S, Safiejko-Mroczka B, Mao C |
| Citation missing |
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| Submission date |
Jul 23, 2010 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Mikhail Dozmorov |
| E-mail(s) |
mdozmorov@vcu.edu
|
| Organization name |
Virginia Commonwealth University
|
| Department |
Biostatistics
|
| Street address |
830 E Main St
|
| City |
Richmond |
| State/province |
VA |
| ZIP/Postal code |
23298 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL6884 |
Illumina HumanWG-6 v3.0 expression beadchip |
|
| Samples (16)
|
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| Relations |
| BioProject |
PRJNA131509 |
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