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Series GSE23136 Query DataSets for GSE23136
Status Public on Aug 24, 2010
Title Genome-wide analysis of time-dependent gene expression in MCF-10HER-2 cells treated with the EGFR-specific inhibitor gefitinib for 45 hours
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Results of blocking activated EGFR/HER-2 heterodimer signaling in MCF-10HER-2 cells by treatment with gefitinib and measuring gene expression as a function of time provides information as to what genes are regulated by EGFR/HER-2 signaling in these breast epithelial cells that are transduced to overexpress the HER-2 oncogene and express transformed phenotypes.
 
Overall design MCF-10HER-2 cells were treated with the EGFR-specific small molecule kinase inhibitor gefitinib for 45 hours. Total RNA was collected every 3 hours from parallel cultures throughout the 45 hour duration of treatment and genome-wide analysis of expression was performed on RNA from each time point (total 16 time points, starting at 0 hours treatment).
 
Contributor(s) Bollig-Fischer A, Ethier SP
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jul 25, 2010
Last update date Mar 22, 2012
Contact name Aliccia B Bollig-Fischer
E-mail(s) bollig@karmanos.org
Organization name Karmanos Cancer Inst., Wayne State University
Street address 4100 John R, HWCRC rm 815
City Detroit
State/province MI
ZIP/Postal code 48201
Country USA
 
Platforms (1)
GPL6255 Illumina humanRef-8 v2.0 expression beadchip
Samples (16)
GSM569959 Gefitinib-treated_MCF10HER2_0hour
GSM569960 Gefitinib-treated_MCF10HER2_3hour
GSM569961 Gefitinib-treated_MCF10HER2_6hour
This SubSeries is part of SuperSeries:
GSE23175 HER-2 signaling, acquisition of growth factor independence, and regulation of biological networks associated with cell transformation
Relations
BioProject PRJNA133239

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE23136_non-normalized.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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