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| Status |
Public on Jul 21, 2011 |
| Title |
Gene expression in TNF treated rat aortic rings cultured in collagen or fibrin gels. |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Angiogenesis in cultures of rat aorta begins with neovessels sprouting from the aortic explant within the first three days of culture.
We used microarrys to examine the effects of TNF-alpha on gene expression in both fibrin and collagen gels during the first 48 hours or culture.
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| Overall design |
Rat aortic rings were cultured in either collagen or fibrin maticies. Half of the cultures from each matrix group were treated with 10ng/ml recombinant rat TNF-alpha, and half were left untreated. These cultures were used to prepare total RNA
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| Contributor(s) |
Ligresti G, Aplin AC, Nicosia RF |
| Citation(s) |
21372301 |
| Submission date |
Jul 26, 2010 |
| Last update date |
Nov 06, 2013 |
| Contact name |
Alfred C Aplin |
| E-mail(s) |
aaplin@u.washington.edu
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| Phone |
206-277-1528
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| Organization name |
University of Washington
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| Department |
Pathology
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| Street address |
Box 357470 HSB-UW
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| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98195 |
| Country |
USA |
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| Platforms (1) |
| GPL6247 |
[RaGene-1_0-st] Affymetrix Rat Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (12)
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| GSM570066 |
Collagen control culture, biological rep1 |
| GSM570067 |
Collagen control culture, biological rep2 |
| GSM570068 |
Collagen control culture, biological rep3 |
| GSM570069 |
TNF treated collagen culture, biological rep1 |
| GSM570070 |
TNF treated collagen culture, biological rep2 |
| GSM570071 |
TNF treated collagen culture, biological rep3 |
| GSM570072 |
Fibrin control culture, biological rep1 |
| GSM570073 |
Fibrin control culture, biological rep2 |
| GSM570074 |
Fibrin control culture, biological rep3 |
| GSM570075 |
TNF treated fibrin culture, biological rep1 |
| GSM570076 |
TNF treated fibrin culture, biological rep2 |
| GSM570077 |
TNF treated fibrin culture, biological rep3 |
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| Relations |
| BioProject |
PRJNA131573 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE23153_RAW.tar |
48.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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