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Series GSE23155 Query DataSets for GSE23155
Status Public on Dec 31, 2013
Title Comparative global transcriptomic profiling of low and high concentration of lactacystin-mediated neuronal death
Organism Mus musculus
Experiment type Expression profiling by array
Summary Inhibition of proteasome degradation pathway has been implicated in neuronal cell death leading to neurodegenerative diseases such as Parkinson’s disease and Alzheimer’s disease. Pharmacological proteasomal inhibitors such as lactacystin can induce apoptosis in cultured mouse cortical neurons through the activation of caspase-3. However, Meiners et al., 2003 suggested that low dose of lactacystin induces a concerted expression of proteasome genes and de novo formation of proteasomes.
Overall design We discovered by microarray analysis that lactacystin treatment induces a dose-dependent biphasic modulation of both potentially neuroprotective as well as pro-apoptotic genes in neurons.
Microarray analysis was carried out using 24 Illumina mouse Ref8V1.1 genechip arrays. The assignment of the arrays was as follows: Controls (n=6); exposure to 1 μM (High) lactacystin for 5h, 8h, 15h and 24 h (n=3 respectively) and 0.1uM (Low) for 15h and 24h (n=3 respectively).
Contributor(s) Cheung N, Chen MJ, Peng ZF
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Submission date Jul 26, 2010
Last update date Dec 31, 2013
Contact name Minghui Jessica Chen
Organization name Menzies Research Institute
Department Neuroscience group
Lab A/P Steve Cheung
Street address Menzies Research Institute, University of Tasmania, Private Bag 24
City Hobart
State/province Tasmania
ZIP/Postal code 7001
Country Australia
Platforms (1)
GPL6103 Illumina mouseRef-8 v1.1 expression beadchip
Samples (24)
GSM570095 Cortical neurons-Ctrl-Rep 1 [H and L Lact]
GSM570096 Cortical neurons-Ctrl-Rep 2 [H and L Lact]
GSM570097 Cortical neurons-Ctrl-Rep 3 [H and L Lact]
BioProject PRJNA131567

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE23155_H_and_LLact_Sample_Gene_Profile.txt.gz 4.9 Mb (ftp)(http) TXT
GSE23155_RAW.tar 3.4 Mb (http)(custom) TAR
GSE23155_non_normalized.txt.gz 2.3 Mb (ftp)(http) TXT
GSE23155_normalized.txt.gz 3.6 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record
Raw data are available on Series record

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