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Series GSE23162 Query DataSets for GSE23162
Status Public on Jan 10, 2011
Title Global transcriptomic profiling of ischemic/reperfusion injury in an in vivo Gpx1 -/- transgenic mouse model.
Organism Mus musculus
Experiment type Expression profiling by array
Summary Glutathione peroxidase (Gpx) is a selenium-containing enzyme that catalyses the reduction of a variety of biological peroxides at the expense of reduced glutathione (GSH). Gpx1 is the most abundant isoform and its role has been implicated in neurodegenerative disorders such as Parkinson’s disease (PD), dementia with Lewy bodies tissue (DLB) (Power and Blumbergs, 2009) and traumatic brain injury (Tsuru-Aoyagi et al., 2009). Due to its high abundance, mutation of the Gpx1 allele would lower overall Gpx activity in the brain significantly. Gpx1 knockout (Gpx1-/-) mice do not show overt phenotypic differences, but all indications suggest that these mice are in a chronic “pro-oxidant” state (Cheng et al. 1999; de Haan et al. 2004). Indeed, a recent study from our laboratory illustrated that the absence of Gpx1 exacerbated stroke injury via increased ROS production and vascular permeability (Wong et al. 2008). Furthermore, Gpx1-/- mice demonstrated an increase in caspase-3 activation and greater infarct volume (Crack et al. 2001)
 
Overall design Microarray analysis was performed on the right striatum and cortex(corresponded to infarct area) of post-I/R injured brain tissues of Gpx1 -/- brains using Illumina mouse Ref8 V2 genechips. Suture-induced middle cerebral artery occlusion was induced for 2h followed by reperfusion, with tissue extraction taking place 2h, 8h and 24h post-reperfusion (n=4 respectively). Sham controls were included in this study too (n=4 respectively).
 
Contributor(s) Cheung NS, Chen MJ, Crack P, Manikandan J
Citation(s) 21193029
Submission date Jul 26, 2010
Last update date Jun 14, 2018
Contact name Minghui Jessica Chen
Organization name Menzies Research Institute
Department Neuroscience group
Lab A/P Steve Cheung
Street address Menzies Research Institute, University of Tasmania, Private Bag 24
City Hobart
State/province Tasmania
ZIP/Postal code 7001
Country Australia
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (32)
GSM570225 Cortex-Sham-Rep1_Gpx1-/-
GSM570226 Cortex-Sham-Rep2_Gpx1-/-
GSM570227 Cortex-Sham-Rep3_Gpx1-/-
This SubSeries is part of SuperSeries:
GSE23163 Global transcriptomic profiling of ischemic/reperfusion injury in an in vivo mouse model.
Relations
BioProject PRJNA133271

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE23162_GPXKO_MCAOStriata_Sample_Probe_Profile.txt.gz 4.4 Mb (ftp)(http) TXT
GSE23162_GPX_KO_MCAOCortex_Sample_Probe_Profile.txt.gz 4.5 Mb (ftp)(http) TXT
GSE23162_RAW.tar 3.1 Mb (http)(custom) TAR
GSE23162_non_normalized.txt.gz 2.3 Mb (ftp)(http) TXT
Processed data included within Sample table
Raw data are available on Series record

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