NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE23289 Query DataSets for GSE23289
Status Public on Feb 01, 2011
Title HUVECs cultured under normal or high laminar shear stress
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Many studies have characterised the effect of normal laminar shear stress (LSS) on endothelial responses, however elevated shear stress, as would be experienced overlying a stenotic plaque, has not been studied in depth. Therefore we used transcriptomics and related functional analyses to compare cells exposed to laminar shear stress at 15 or 75 dynes/cm2 for 24 hours (LSS15-normal or LSS75-high shear stress).
 
Overall design Human umbilical vein endothelial cells (HUVEC n=4 per flow condition from different batches of pooled donor HUVEC p2) were cultured for 24 hours on gelatin coated slides under laminar shear stress of either 15 dynes/cm2 or 75 dynes/cm2 (LSS15 or LSS75) to assess the effect of high shear stress on endothelial cells. Total RNA was obtained using Qiagen kit and array analysis performed by Service XS (Leiden, Netherlands).
 
Contributor(s) White S
Citation(s) 21302282
Submission date Jul 28, 2010
Last update date Jan 18, 2013
Contact name Stephen John White
E-mail(s) steve.white@bris.ac.uk
Phone +44 (0)117 3423586
Organization name University of Bristol
Department Bristol Heart Institute
Street address Level 7 Queens Building, Bristol Royal Infirmary
City Bristol
ZIP/Postal code BS2 8HW
Country United Kingdom
 
Platforms (1)
GPL6104 Illumina humanRef-8 v2.0 expression beadchip
Samples (8)
GSM591781 HUVEC_LSS15_rep1
GSM591782 HUVEC_LSS15_rep2
GSM591783 HUVEC_LSS15_rep3
Relations
BioProject PRJNA131365

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE23289_RAW.tar 3.4 Mb (http)(custom) TAR
GSE23289_non-normalized.txt.gz 1.3 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap