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Status |
Public on Feb 01, 2011 |
Title |
HUVECs cultured under normal or high laminar shear stress |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Many studies have characterised the effect of normal laminar shear stress (LSS) on endothelial responses, however elevated shear stress, as would be experienced overlying a stenotic plaque, has not been studied in depth. Therefore we used transcriptomics and related functional analyses to compare cells exposed to laminar shear stress at 15 or 75 dynes/cm2 for 24 hours (LSS15-normal or LSS75-high shear stress).
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Overall design |
Human umbilical vein endothelial cells (HUVEC n=4 per flow condition from different batches of pooled donor HUVEC p2) were cultured for 24 hours on gelatin coated slides under laminar shear stress of either 15 dynes/cm2 or 75 dynes/cm2 (LSS15 or LSS75) to assess the effect of high shear stress on endothelial cells. Total RNA was obtained using Qiagen kit and array analysis performed by Service XS (Leiden, Netherlands).
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Contributor(s) |
White S |
Citation(s) |
21302282 |
Submission date |
Jul 28, 2010 |
Last update date |
Jan 18, 2013 |
Contact name |
Stephen John White |
E-mail(s) |
steve.white@bris.ac.uk
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Phone |
+44 (0)117 3423586
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Organization name |
University of Bristol
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Department |
Bristol Heart Institute
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Street address |
Level 7 Queens Building, Bristol Royal Infirmary
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City |
Bristol |
ZIP/Postal code |
BS2 8HW |
Country |
United Kingdom |
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Platforms (1) |
GPL6104 |
Illumina humanRef-8 v2.0 expression beadchip |
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Samples (8)
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Relations |
BioProject |
PRJNA131365 |