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Series GSE23317 Query DataSets for GSE23317
Status Public on Dec 31, 2013
Title Global transcriptomic profiling of hypoxic ischemia in an in vivo neonatal mouse model: cortex
Organism Mus musculus
Experiment type Expression profiling by array
Summary Hypoxia-ischemia (HI) brain damage is one of the most common causes of neonatal brain injuries, amidst other conditions such as intrauterine infection and perinatal cerebral hemorrhage (Bracci et al., 2006). HI, occurring during the perinatal period, severely affects brain integrity resulting in detrimental long-term neurological morbidity in terms of motor, intellectual, educational and neuropsychological performance deficits (e.g. cerebral palsy, mental retardation, learning disability and epilepsy), and even neonatal mortality (Cowan et al., 2003; Ferriero, 2004; van Handel et al., 2007; Shalak and Perlman, 2004). Current therapeutic interventions fail to provide substantial reversal of HI brain injuries and improvement in overall cognitive function. Recent clinical studies demonstrated that post-HI hypothermia provide moderate neuroprotection but fail to show any significant reduction in neonatal morbidity and mortality (Shankaran et al., 2005). We would like to investigate the transcriptional effects of HI on neonatal brain, and if hypoxic pre-conditioning is beneficial to the reduction of brain damage.
 
Overall design Microarray analysis was performed on the cortex of neonatal brains using Illumina mouse Ref8 V2 genechips. The right common carotid artery was exposed through a ventral midline neck incision and permanently occluded by electrocoagulation, The wound was sutured and mouse pups were returned to their mother for 1.5–2 h. Sham control and pre-conditioned mice (n = 4) underwent the identical procedure, without carotid artery occlusion. Pups were then placed in an 8% O2/92% N2 humidified chamber at 37°C for 1 h with tissue extraction taking place 3h, 8h and 24h thereafter (n=4 respectively). Sham controls were included in this study too (n=4 respectively).
 
Contributor(s) Cheung NS, Chen M, Crack P, Manikandan J
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jul 29, 2010
Last update date Jun 14, 2018
Contact name Minghui Jessica Chen
Organization name Menzies Research Institute
Department Neuroscience group
Lab A/P Steve Cheung
Street address Menzies Research Institute, University of Tasmania, Private Bag 24
City Hobart
State/province Tasmania
ZIP/Postal code 7001
Country Australia
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (59)
GSM572174 Cortex-Sham-0h-Rep1
GSM572175 Cortex-Sham-0h-Rep2
GSM572176 Cortex-Sham-0h-Rep3
This SubSeries is part of SuperSeries:
GSE23333 Global transcriptomic profiling of hypoxic ischemia in an in vivo neonatal mouse model
Relations
BioProject PRJNA133301

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE23317_HI&HP8hStraitum_Cortex_Sample_Probe_Profile.txt.gz 8.6 Mb (ftp)(http) TXT
GSE23317_HI0h&3h_Sample_Probe_Profile copy.txt.gz 17.5 Mb (ftp)(http) TXT
GSE23317_HI24hStriatum_Sample_Probe_Profile.txt.gz 4.5 Mb (ftp)(http) TXT
GSE23317_HI_HP24hCortex_Sample_Probe_Profile.txt.gz 4.5 Mb (ftp)(http) TXT
GSE23317_RAW.tar 3.1 Mb (http)(custom) TAR
GSE23317_non-normalized.txt.gz 3.6 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table
Processed data are available on Series record

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