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Series GSE23381 Query DataSets for GSE23381
Status Public on Aug 03, 2010
Title Transcription profile of zebrafish hand2 (hanS6 allele) mutant embryos at 19hpf
Organism Danio rerio
Experiment type Expression profiling by array
Summary Heart formation requires the fusion of bilateral cardiomyocyte populations as they move toward the embryonic midline. The bHLH transcription factor Hand2 is essential for cardiac fusion; however, the effector genes that execute this function of Hand2 are unknown. Here, we provide the first evidence for a downstream component of the Hand2 pathway that mediates cardiac morphogenesis. Although hand2 is expressed in cardiomyocytes, mosaic analysis demonstrates that hand2 plays a non-autonomous role in regulating cardiomyocyte movement. Gene expression profiles reveal heightened expression of fibronectin 1 (fn1) in hand2 mutant embryos. Reciprocally, overexpression of hand2 leads to decreased Fibronectin levels. Furthermore, reduction of fn1 function enables rescue of cardiac fusion in hand2 mutants: bilateral cardiomyocyte populations merge and exhibit improved tissue architecture, albeit without major changes in apicobasal polarity. Together, our data provide a novel example of a tissue creating a favorable environment for its morphogenesis: the Hand2 pathway establishes an appropriate environment for cardiac fusion through negative modulation of Fn1 levels.
 
Overall design Embryos from three independent hand2 mutant (hanS6 allele) heterozyogous crosses were collected. Examination of Tg(myl7:egfp) expression allow sorting of hand2 mutant embryos from their wild-type siblings.
 
Contributor(s) Garavito-Aguilar ZV, Yelon D
Citation(s) 20724450
Submission date Aug 02, 2010
Last update date Jan 25, 2018
Contact name Zayra Viviana Garavito-Aguilar
E-mail(s) zgaravitoaguilar@ucsd.edu
Fax (858)534-0555
Organization name University of California, San Diego
Department Division of Biological Sciences
Lab Yelon Lab
Street address 9500 Gilman Drive #0347
City La Jolla
State/province CA
ZIP/Postal code 92093-0347
Country USA
 
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (6)
GSM573573 zebrafish_han_WT_Rep1
GSM573574 zebrafish_han_Mut_Rep1
GSM573575 zebrafish_han_WT_Rep2
Relations
BioProject PRJNA131267

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE23381_RAW.tar 20.8 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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