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| Status |
Public on Sep 09, 2010 |
| Title |
Prdm16 newborn mouse (ventricular zone) VZ cells microarray |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
As Prdm16 deficiency reduces self-renewal potential and depletes neural stem cells in culture we decided to investigate the underlying molecular mechanisms of the neural stem cells depletion in the Prdm16 deficient animals. For the experiment we used Prdm16Gt(OST67423)Lex (Prdm16LacZ) genetrap mice obtained from the NIH Mutant Mouse Regional Resource Center (http://www.mmrrc.org/).
We compared the gene expression profiles of uncultured ventricular zone cells from newborn Prdm16LacZ/LacZ (KO), Prdm16LacZ/+(HET), and Prdm16+/+ (WT) mice.
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| Overall design |
The uncultured ventricular zone cells from newborn Prdm16LacZ/LacZ, Prdm16LacZ/+, and Prdm16+/+ mice were dissected from the brains and placed into the Trizol reagent. Purified RNA was reverse transcribed and amplified using the WT-Ovation™ Applause WT/Amp RNA amplification system (NuGEN Technologies,) following the manufacturer’s instructions. Sense strand cDNA was fragmented and labeled using the FL-Ovation™ cDNA Biotin Module V2 (NuGEN). 2.5µg of labeled cDNA were hybridized to Affymetrix Mouse Gene ST 1.0 microarrays.
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| Contributor(s) |
Chuikov S, Levi BP, Morrison SJ |
| Citation(s) |
20835244 |
| Submission date |
Aug 04, 2010 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Sean J Morrison |
| E-mail(s) |
seanjm@umich.edu
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| Phone |
734-647-6261
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| Organization name |
Center for Stem Cell Biology, 5435 Life Sciences Institute University of Michigan
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| Department |
HHMI/Department of Internal Medicine
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| Street address |
210 Washtenaw Ave
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| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109-2216 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA131057 |
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