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| Status |
Public on Dec 01, 2010 |
| Title |
Indian Hedgehog Signaling in the Postnatal Growth Plate |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
In order to characterize mRNA expression in the growth plate, we microdissected postnatal rat growth plates into their constituent zones and used microarray analysis to assess the abundences of individual transcripts. Expression patterns of PTHrP and Ihh-related genes were confirmed using real-time PCR. Using a gli1-lacZ mouse, Gli1 expression, presumably representing Ihh signaling, was visualized during pre- and postnatal development.
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| Overall design |
Microdissection was used to collect individual growth plate zones from proximal tibiae of 1-wk rats and gene expression was analyzed using microarray.
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| Contributor(s) |
Chau M, Forcinito P, Andrade AC, Hegde A, Ahn S, Lui JC, Baron J, Nilsson O |
| Citation(s) |
21642420 |
| Submission date |
Aug 05, 2010 |
| Last update date |
Jul 31, 2017 |
| Contact name |
Ola Nilsson |
| E-mail(s) |
ola.nilsson@ki.se
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| URL |
http://ki.se/ki/jsp/polopoly.jsp?d=17187&l=en
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| Organization name |
Karolinska Institute
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| Department |
Dept. Woman and Child Health
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| Lab |
Cartilage and Bone Biology
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| Street address |
Karolinska University Hospital, Q2:08
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| City |
Stockholm |
| ZIP/Postal code |
17176 |
| Country |
Sweden |
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| Platforms (1) |
| GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
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| Samples (24)
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| GSM425049 |
Resting zone, Biol rep 4 |
| GSM425050 |
Resting zone, Biol rep 5 |
| GSM425051 |
Proliferative zone, Biol rep 1 |
| GSM425052 |
Proliferative zone, Biol rep 2 |
| GSM425053 |
Proliferative zone, Biol rep 3 |
| GSM425054 |
Proliferative zone, Biol rep 4 |
| GSM425055 |
Proliferative zone, Biol rep 5 |
| GSM425056 |
Hypertrophic zone, Biol rep 1 |
| GSM425057 |
Hypertrophic zone, Biol rep 2 |
| GSM425058 |
Hypertrophic zone, Biol rep 3 |
| GSM425059 |
Hypertrophic zone, Biol rep 4 |
| GSM425060 |
Hypertrophic zone, Biol rep 5 |
| GSM575172 |
Epiphyseal cartilage, Biol rep 1 |
| GSM575173 |
Epiphyseal cartilage, Biol rep 2 |
| GSM575174 |
Epiphyseal cartilage, Biol rep 4 |
| GSM575175 |
Epiphyseal cartilage, Biol rep 5 |
| GSM575176 |
Proliferative-hypertrophic transition zone, Biol rep 1 |
| GSM575177 |
Proliferative-hypertrophic transition zone, Biol rep 2 |
| GSM575178 |
Proliferative-hypertrophic transition zone, Biol rep 3 |
| GSM575179 |
Proliferative-hypertrophic transition zone, Biol rep 4 |
| GSM575180 |
Proliferative-hypertrophic transition zone, Biol rep 5 |
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| Relations |
| BioProject |
PRJNA131101 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE23432_RAW.tar |
58.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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